Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells
| Autor: | Jody Gorman, Lauren E. Hartley-Tassell, Christopher J. Day, Anuk Indraratna, Mark J. Walker, Martina L. Sanderson-Smith, Stephan Brouwer, Liisa Kautto, Michael P. Jennings, David M. P. De Oliveira, Arun V. Everest-Dass, Nicolle H. Packer, Ailish Cleary |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Glycan Glycosylation Streptococcus pyogenes In Vitro Techniques Biochemistry Fucose Bacterial Adhesion Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Human Glycan Antigen Polysaccharides ABO blood group system Streptococcal Infections Genetics Humans Salivary Proteins and Peptides Molecular Biology chemistry.chemical_classification Antigens Bacterial biology Host Microbial Interactions Virulence Epithelial Cells Sialic acid 030104 developmental biology chemistry biology.protein Blood Group Antigens Glycoprotein Carrier Proteins 030217 neurology & neurosurgery Biotechnology Bacterial Outer Membrane Proteins Protein Binding |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 33(10) |
| ISSN: | 1530-6860 |
| Popis: | Colonization of the oropharynx is the initial step in Group A Streptococcus (GAS) pharyngeal infection. We have previously reported that the highly virulent M1T1 GAS clone attaches to oral epithelial cells via M1 protein interaction with blood group antigen carbohydrate structures. Here, we have identified that colonization of human oral epithelial cells by GAS serotypes M3 and M12 is mediated by human blood group antigens [ABO(H)] and Lewis (Le) antigen expression. Removal of linkage-specific fucose, galactose, N-acetylgalactosamine, and sialic acid modulated GAS colonization, dependent on host ABO(H) blood group and Le expression profile. Furthermore, N-linked glycans from human salivary glycoproteins, when released and purified, were potent inhibitors of M1, M3, and M12 GAS colonization ex vivo. These data highlight the important role played by human protein glycosylation patterns in GAS attachment to oral epithelial cell surfaces.-De Oliveira, D. M. P., Everest-Dass, A., Hartley-Tassell, L., Day, C. J., Indraratna, A., Brouwer, S., Cleary, A., Kautto, L., Gorman, J., Packer, N. H., Jennings, M. P., Walker, M. J., Sanderson-Smith, M. L. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells. |
| Databáze: | OpenAIRE |
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