Mechanism of Mesoporous Silica Nanoparticle Interaction with Hairy Root Cultures during Nanoharvesting of Biomolecules
| Autor: | Stephen E. Rankin, Madeleine Fugate, Dennis T. Rogers, Barbara L. Knutson, John M. Littleton, Jatinder Sambi, M. Arif Khan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Biomolecule Biomedical Engineering Nanoparticle 02 engineering and technology Mesoporous silica Plants 021001 nanoscience & nanotechnology Plant cell Silicon Dioxide General Biochemistry Genetics and Molecular Biology Biomaterials Cell wall 03 medical and health sciences chemistry Plant Cells Hairy root culture Biophysics Surface modification Nanoparticles Nanocarriers 0210 nano-technology 030304 developmental biology |
| Zdroj: | Advanced biology. 5(3) |
| ISSN: | 2701-0198 |
| Popis: | Cellular uptake and expulsion mechanisms of engineered mesoporous silica nanoparticles (MSNPs) are important in their design for novel biomolecule isolation and delivery applications such as nanoharvesting, defined as using nanocarriers to transport and isolate valuable therapeutics (secondary metabolites) out of living plant organ cultures (e.g., hairy roots). Here, temperature-dependent MSNP uptake and recovery processes in hairy roots are examined as a function of surface chemistry. MSNP uptake into hairy roots and time-dependent expulsion are quantified using Ti content (present for biomolecule binding) and fluorescence spectroscopy of fluorescently tagged MSNPs, respectively. The results suggest that functionalization and surface charge (regulated by amine group attachment) play the biggest role in the effectiveness of uptake and recovery. Comparison of MSNP interactions with hairy roots at 4 and 23 °C shows that weakly charged MSNPs functionalized only with Ti are taken up and expelled by thermally activated mechanisms, while amine-modified positively charged particles are taken up and expelled mainly by direct penetration of cell walls. Amine-functionalized MSNPs move spontaneously in and out of plant cells by dynamic exchange with a residence time of 20 ± 5 min, suggesting promise as a biomolecule nanoharvesting platform for plant organ cultures. |
| Databáze: | OpenAIRE |
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