Cell shape information is transduced through tension-independent mechanisms
| Autor: | Evren U. Azeloglu, Rhodora Cristina Calizo, Susana R. Neves-Zaph, Gomathi Jayaraman, Hong Li, James Hone, Sunwoo Lee, John Cijiang He, Ronald E. Gordon, Yibang Chen, Amit Ron, Ravi Iyengar, Smiti Bhattacharya, Mufeng Hu |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell physiology Proteomics Science Cell Myocytes Smooth Muscle General Physics and Astronomy 02 engineering and technology Mechanotransduction Cellular General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Transduction (genetics) Chlorocebus aethiops medicine Myocyte Animals Humans Mechanotransduction lcsh:Science Cell Shape Cells Cultured Multidisciplinary COS cells Chemistry Podocytes Microfilament Proteins Integrin beta3 Membrane Proteins General Chemistry Fibroblasts 021001 nanoscience & nanotechnology Phenotype Cell biology Rats Cytoskeletal Proteins 030104 developmental biology medicine.anatomical_structure Animals Newborn COS Cells lcsh:Q Stress Mechanical Signal transduction 0210 nano-technology |
| Zdroj: | Nature Communications Nature Communications, Vol 8, Iss 1, Pp 1-15 (2017) |
| ISSN: | 2041-1723 |
| Popis: | The shape of a cell within tissues can represent the history of chemical and physical signals that it encounters, but can information from cell shape regulate cellular phenotype independently? Using optimal control theory to constrain reaction-diffusion schemes that are dependent on different surface-to-volume relationships, we find that information from cell shape can be resolved from mechanical signals. We used microfabricated 3-D biomimetic chips to validate predictions that shape-sensing occurs in a tension-independent manner through integrin β3 signaling pathway in human kidney podocytes and smooth muscle cells. Differential proteomics and functional ablation assays indicate that integrin β3 is critical in transduction of shape signals through ezrin–radixin–moesin (ERM) family. We used experimentally determined diffusion coefficients and experimentally validated simulations to show that shape sensing is an emergent cellular property enabled by multiple molecular characteristics of integrin β3. We conclude that 3-D cell shape information, transduced through tension-independent mechanisms, can regulate phenotype. It is not known whether the shape of a cell can regulate cellular phenotype independently. Here, the authors show that culturing kidney podocytes or smooth muscle cells on 3-D biomimetic surfaces results in phenotypic changes and that cell shape is sensed by integrin β3 in a tension-independent manner. |
| Databáze: | OpenAIRE |
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