Multicenter Evaluation of Strategies for Serodiagnosis of Primary Infection with Toxoplasma gondii
| Autor: | Darrel O Ho-Yen, E. Petersen, Edward Guy, G. Enders, Anne Decoster, A Roberts, R-M Blatz, M-H Bessières, Klaus Hedman, Anne Naessens, Ermanno Candolfi, Michael Hayde, B. Lecolier, J. Zufferey, Hervé Pelloux, J. D. Johnson, U Gross, Philippe Thulliez, V. Luyasu |
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| Přispěvatelé: | Immunology and Microbiology, Vrije Universiteit Brussel |
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Immunoglobulin A Microbiology (medical) media_common.quotation_subject Antibody Affinity Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Immunoglobulin G Serology Antibody Specificity Pregnancy medicine Animals Humans Serologic Tests Aged media_common biology Convalescence Toxoplasma gondii General Medicine Middle Aged biology.organism_classification medicine.disease Virology Toxoplasmosis Infectious Diseases Immunoglobulin M Acute Disease Immunology biology.protein Female Antibody Toxoplasma Follow-Up Studies |
| Zdroj: | ResearcherID |
| ISSN: | 0934-9723 |
| DOI: | 10.1007/s100960100543 |
| Popis: | The diagnostic performance of single-serum assays for toxoplasma-specific immunoglobulin (Ig)M. IgA. IgG, and IgE antibodies and of different combinations of such antibody assays in 20 European reference centers was assessed. A panel of 276 sera, of which 73 came from patients who seroconverted within 3 months (acute infection), 49 from patients who had seroconverted 3-12 months earlier (convalescence), and 154 from subjects who had two IgG-positive samples obtained 12 months apart (past infection), was tested with 20 toxoplasma-antibody assays and 195 combinations. In general, every assay with high diagnostic sensitivity showed low diagnostic specificity, i.e. no assay performed alone could reliably distinguish acute from past infection. Furthermore, no single assay (or combination) could separate convalescence from the other stages of toxoplasma infection. However, excellent diagnostic performances were reached by sequential use of highly sensitive IgM assays and methods examining IgG avidity or stage specificity. IgA or IgM assays were less suitable for confirmation of toxoplasma-IgM positivity. This study documents the strength of test combinations in assessing the stage of toxoplasma infection. |
| Databáze: | OpenAIRE |
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