On the sub-maximal yield and photo-electric stimulation of chlorophyll a fluorescence in single turnover excitations in plant cells
| Autor: | Gustavo Costa Rodrigues, Jack J. S. van Rensen, Wim J. Vredenberg |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chlorophyll
Chlorophyll a donor-side Photosystem II Photochemistry Biophysics Analytical chemistry reduction in-vivo Fluorescence Chenopodium album chemistry.chemical_compound Electricity spinach thylakoids Electrochemistry Laboratorium voor Plantenfysiologie Physical and Theoretical Chemistry induction Chlorophyll fluorescence Quenching (fluorescence) Chlorophyll A EPS-3 illumination DCMU thylakoid membrane General Medicine Electric Stimulation Plant Leaves Kinetics Spectrometry Fluorescence chemistry photosystem-ii chloroplasts Yield (chemistry) Thylakoid flash Laboratory of Plant Physiology |
| Zdroj: | Bioelectrochemistry 68 (2006) 1 Bioelectrochemistry, 68(1), 81-88 |
| ISSN: | 1567-5394 |
| Popis: | A set of expressions is derived which quantifies the chlorophyll fluorescence yield in terms of rate constants of primary light reactions of PSII, the fraction of open and semi-open RCs and of the electric field sensed by the RC in the thylakoid membrane. The decay kinetics of the chlorophyll fluorescence yield after a single turnover excitation in the presence of DCMU show at least two components, one reversible within approx. 1 s and one with a dark reversion lasting more than 30 s. The latter is attributed to photochemical quenching; the fast component is interpreted to be associated at least partially with photo-electrochemical control. It will be illustrated that (i) the sub-maximal fluorescence yield in single turnover excitation is associated with semi-closure of RCs, (ii) the trapping efficiency of semi-closed centers is less than 50% of that of open centers and (iii) the fluorescence yield of antennas with semi-closed RCs has the highest sensitivity to changes in strength of photo-electric fields. |
| Databáze: | OpenAIRE |
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