Secretion of Gut Hormones and Expression of Sweet Taste Receptors and Glucose Transporters in a Rat Model of Obesity
| Autor: | Qian Jing Liu, Lian Yong Liu, Wei Liu, Jing Ma, Cheng Qian, Yun Qiu Jin, Sheng Xian Li, Ri Lu Feng, Christopher K. Rayner |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Health (social science) medicine.medical_treatment Glucose Transport Proteins Facilitative Gene Expression Receptors G-Protein-Coupled Rats Sprague-Dawley 0302 clinical medicine Glucagon-Like Peptide 1 Insulin Receptor lcsh:RC620-627 digestive oral and skin physiology Taste Perception lcsh:Nutritional diseases. Deficiency diseases medicine.anatomical_structure Hypothalamus Taste lcsh:Nutrition. Foods and food supply hormones hormone substitutes and hormone antagonists Research Article medicine.medical_specialty 030209 endocrinology & metabolism Ileum lcsh:TX341-641 Sweet taste receptors Diet High-Fat Gastrointestinal Hormones 03 medical and health sciences Physiology (medical) Internal medicine medicine Animals Peptide YY Obesity 030109 nutrition & dietetics business.industry Glucose transporter Glucose Tolerance Test Small intestine Gut hormones Rats Endocrinology Glucose Duodenum business Hormone |
| Zdroj: | Obesity Facts, Vol 12, Iss 2, Pp 190-198 (2019) |
| ISSN: | 1662-4033 |
| Popis: | Objectives: This study was undertaken to compare gut hormone secretion between high-fat-fed and control rats, and to examine the corresponding changes in the expression of sweet taste receptors and glucose transporters in the small intestine and hypothalamus. Methods: Four-week-old male Sprague Dawley rats were fed a standard or high-fat diet for 8 weeks (10 in each group), followed by an oral glucose tolerance test (50% glucose solution, 2 g/kg). Blood was sampled for glucose, insulin, glucagon-like peptide-1 (GLP-1) and polypeptide YY (PYY) assays. One week later, small intestinal and hypothalamic tissue were analyzed for sweet taste receptor and glucose transporter expression by real-time PCR. Results: After oral glucose, plasma GLP-1 concentrations were higher in high-fat-fed than standard-fat-fed rats (group × time interaction, p < 0.01) with significant differences at t = 15 min (p < 0.01) and 30 min (p < 0.05). Plasma PYY concentrations were lower in high-fat-fed than control rats at t = 0, 15 min (p < 0.05, respectively) and 120 min (p < 0.01). There were no differences in the expression of sweet taste receptors or glucose transporters between high-fat-fed and control rats in the duodenum, ileum, or hypothalamus. Conclusions: Changes in GLP-1 and PYY secretion after a high-fat diet appear unrelated to any changes in the expression of sweet taste receptors or glucose transporters. Impaired PYY secretion with high-fat feeding suggests that PYY analogues may provide a potential therapy in the treatment of obesity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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