Sulforaphane Modifies Histone H3, Unpacks Chromatin, and Primes Defense
| Autor: | Stephani Baum, Britta Schillheim, Carsten Bolm, Uwe Conrath, Irina Jansen, Alexander Beesley |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Arabidopsis Plant Science 01 natural sciences Histones 03 medical and health sciences chemistry.chemical_compound Histone H3 Gene Expression Regulation Plant Isothiocyanates Phytoalexins Genetics Signaling and Response Mode of action Promoter Regions Genetic Hyaloperonospora arabidopsidis biology Arabidopsis Proteins Lysine fungi food and beverages biology.organism_classification Chromatin Cell biology High-Throughput Screening Assays 030104 developmental biology chemistry Oomycetes Sulfoxides Isothiocyanate Host-Pathogen Interactions H3K4me3 Petroselinum Oxidation-Reduction Sesquiterpenes Sulfur 010606 plant biology & botany Sulforaphane Transcription Factors |
| Zdroj: | Plant physiology. 176(3) |
| ISSN: | 1532-2548 |
| Popis: | Modern crop production calls for agrochemicals that prime plants for enhanced defense. Reliable test systems for spotting priming-inducing chemistry, however, are rare. We developed an assay for the high-throughput search for compounds that prime microbial pattern-induced secretion of antimicrobial furanocoumarins (phytoalexins) in cultured parsley cells. The screen produced 1-isothiocyanato-4-methylsulfinylbutane (sulforaphane; SFN), a secondary metabolite in many crucifers, as a novel defense priming compound. While elucidating SFN’s mode of action in defense priming, we found that in Arabidopsis (Arabidopsisthaliana) the isothiocyanate provokes covalent modification (K4me3, K9ac) of histone H3 in the promoter and promoter-proximal region of defense genes WRKY6 and PDF1.2, but not PR1. SFN-triggered H3K4me3 and H3K9ac coincide with chromatin unpacking in the WRKY6 and PDF1.2 regulatory regions, primed WRKY6 expression, unprimed PDF1.2 activation, and reduced susceptibility to downy mildew disease (Hyaloperonospora arabidopsidis). Because SFN also directly inhibits H. arabidopsidis and other plant pathogens, the isothiocyanate is promising for the development of a plant protectant with a dual mode of action. |
| Databáze: | OpenAIRE |
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