Development of nuclear DNA probes for the typing of Trypanosoma cruzi
| Autor: | Yara M. Traub-Cseko, Takeuchi Am |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Microbiology (medical)
Genetic Markers lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 Trypanosoma cruzi lcsh:QR1-502 Isozyme lcsh:Microbiology strains Pulsed-field gel electrophoresis Animals Typing random probes Genetics Genomic Library biology typing DNA Protozoan biology.organism_classification Molecular biology Nuclear DNA Electrophoresis Gel Pulsed-Field Blot Blotting Southern Genetic marker numerical taxonomy Restriction fragment length polymorphism DNA Probes |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 86, Iss 3, Pp 307-315 (1991) |
| ISSN: | 0074-0276 |
| Popis: | We have developed and tested a new way of typing Trypanosoma cruzi, namely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Southern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis and a higher degree of heterogeneity was observed at this level. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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