Salmonella Pathogenicity Island 1 Is Expressed in the Chicken Intestine and Promotes Bacterial Proliferation
Autor: | Lydia M. Bogomolnaya, Michael I. Betteken, L. Garry Adams, Helene Andrews-Polymenis, Colleen R. Eade, Chien Che Hung, Craig Altier |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Salmonella animal structures Salmonella enteritidis 030106 microbiology Immunology Mutant Wild type Virulence Bacterial Infections Biology biochemical phenomena metabolism and nutrition medicine.disease_cause Microbiology Pathogenicity island Type three secretion system 03 medical and health sciences 030104 developmental biology Infectious Diseases medicine Parasitology Colonization |
Popis: | Salmonella enterica serovar Enteritidis is a common cause of foodborne illness in the United States. The bacterium can be transmitted to humans via contaminated chicken meat and eggs, and virulence in humans requires type III secretion system 1 (TTSS-1), encoded on Salmonella pathogenicity island 1 (SPI-1). Chickens often carry S. Enteritidis subclinically, obscuring the role of SPI-1 in facilitating bacterial colonization. To evaluate the role of SPI-1 in the infection of chicks by Salmonella, we created and utilized strains harboring a stable fluorescent reporter fusion designed to quantify SPI-1 expression within the intestinal tracts of animals. Using mutants unable to express TTSS-1, we demonstrated the important role of the secretion system in facilitating bacterial colonization. We further showed that coinoculation of an SPI-1 mutant with the wild-type strain increased the number of mutant organisms in intestinal tissue and contents, suggesting that the wild type rescues the mutant. Our results support the hypothesis that SPI-1 facilitates S. Enteritidis colonization of the chicken and make SPI-1 an attractive target in preventing Salmonella carriage and colonization in chickens to reduce contamination of poultry meat and eggs by this foodborne pathogen. |
Databáze: | OpenAIRE |
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