Paraquat-activated BV-2 microglia induces neuroinflammatory responses in the neuron model through NF-κB signaling pathway
| Autor: | Min Huang, Kaidong Wang, Tian Tian, Huifang Yang, Weiguang Yan, Jian Sun, Yifan Wang, Bingyang Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Paraquat
0301 basic medicine Apoptosis Inflammation Toxicology Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Secretion Cells Cultured Neurons Microglia Herbicides Chemistry NF-kappa B Neurotoxicity NF-κB General Medicine medicine.disease In vitro Rats Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Animals Newborn nervous system 030220 oncology & carcinogenesis Neuron medicine.symptom Signal Transduction |
| Zdroj: | Toxicology in Vitro. 72:105076 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2021.105076 |
| Popis: | Paraquat (PQ), a non-selective contact herbicide, has been generally accepted as one of the environmental neurotoxicants. Despite the direct evidence that PQ could induce inflammation responses in microglia, little is known about the effects of the inflammatory microglia on neurons. Thus in the present study, mouse primary cortical neurons and PC12 cells, widely-used in vitro neuron models for neurotoxicity research were applied to investigate the neuroinflammatory effects of PQ-activated microglia on neurons. We observed that the secretion levels of TNF-α and IL-6 in PC12 cells were markedly increased upon treatment with the supernatants of inflammatory BV2 microglia, and NF-κB p65 protein expression was also elevated. Specific inhibition of NF-κB by PDTC dramatically attenuated the increase of TNF-α and IL-6 release. These results suggested that PQ-induced inflammatory microglia exerts secondary inflammatory effects on neurons through activation of NF-κB pathway. |
| Databáze: | OpenAIRE |
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