Baculovirus structural polypeptides
| Autor: | Gale E. Smith, Max D. Summers |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
biology
Molecular mass viruses fungi Nuclear Polyhedrosis Virus Insect Viruses biochemical phenomena metabolism and nutrition biology.organism_classification Virology Molecular biology Virus Molecular Weight Viral Proteins Autographa californica Capsid Rachiplusia ou Viral envelope Peptides Polyacrylamide gel electrophoresis |
| Zdroj: | Virology. 84:390-402 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(78)90257-x |
| Popis: | The structural polypeptides of eight insect baculoviruses were studied using vertical slab polyacrylamide gel electrophoresis. All viruses revealed a complex but unique composition of 15 to 25 bands with molecular weights ranging from 15,000 to 160,000. Since certain baculoviruses have more than one nucleocapsid per viral envelope (multiples), comparisons were made of the multiples and singles (enveloped single nucleocapsids) for each virus. Quantitative and qualitative differences were documented to exist in polypeptide composition. Where possible, the envelopes of certain baculoviruses were selectively removed in order to identify the major capsid proteins. Autographa californica M NPV (NPV: nuclear polyhedrosis virus) capsids contained two major polypeptides, VP18.5 and VP37. Rachiplusia ou M NPV capsids contained several polypeptides of which VP16, VP17, VP18, VP30, and VP36 were considered major constituents. Anticarsa gemmatalis M NPV contained one major capsid protein, VP29, and several minor polypeptides. Major capsid proteins of Heliothis zea SNPV were VP16, VP28, and VP63; as were VP16, VP17, and VP31 of Trichoplusia ni granulosis virus (GV). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|