Frequency and Types of Point Mutation at the 12th Codon of the c‐Ki‐ras Gene Found in Pancreatic Cancers from Japanese Patients
| Autor: | Kozo Nakamura, Mariko Mariyama, Susumu Nishimura, Kiyozo Kishi, Hiroshi Obata |
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| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
Silent mutation
Cancer Research c‐Ki‐ras Nonsense mutation Biology Adenocarcinoma medicine.disease_cause digestive system Polymerase Chain Reaction Gene Frequency Japan medicine Missense mutation Humans Codon Gene Pancreas Genetics Mutation Transition (genetics) Base Sequence Point mutation Nucleic acid sequence DNA Neoplasm digestive system diseases Pancreatic Neoplasms Genes ras Oncology Oligonucleotide Probes Rapid Communication |
| Zdroj: | Japanese Journal of Cancer Research : Gann |
| ISSN: | 1876-4673 0910-5050 |
| Popis: | Point mutations at the 12th codon of c-Ki-ras in pancreatic cancer from Japanese patients were examined using the polymerase chain reaction, followed by cloning of the amplified gene fragments in pTZ phagemid and nucleotide sequence determination. The frequency of the point mutations found in the tumors was quite high (75%). The mutation most frequently detected was a G---A transition at the second position of codon 12 (GGT---GAT), but other types of mutations such as GGT----GTT and GGT----CGT were also found. In one case, silent mutation of GGT to GGC was detected in addition to the frequent mutation of GGT to GAT. These observations suggest that the 12th codon of pancreatic c-Ki-ras is highly mutatable. |
| Databáze: | OpenAIRE |
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