Resistance to P. brasiliensis experimental infection of inbred mice is associated with an efficient neutrophil mobilization and activation by mediators of inflammation
| Autor: | Gisele Pesquero Fernandes, Eva Burger, Giulia M. A. C. Bani, Ana Carolina S. C. Mendes, Vera Lúcia Garcia Calich, Felipe Fornias Sperandio |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Article Subject Neutrophils Immunology Inflammation INFLAMAÇÃO Biology Neutrophil Activation Proinflammatory cytokine Mice Immune system Bone Marrow Cell Movement medicine lcsh:Pathology Animals Pathogen Colony-forming unit Paracoccidioidomycosis Paracoccidioides Cell Biology medicine.disease Interleukin-10 Interleukin 10 medicine.anatomical_structure Bone marrow Inflammation Mediators medicine.symptom Research Article lcsh:RB1-214 |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Mediators of Inflammation, Vol 2015 (2015) Mediators of Inflammation |
| Popis: | Paracoccidioidomycosis (PCM) is a systemic fungal infection, endemic in Brazil, that leads to severe morbidity and even mortality if not correctly treated. Patients may respond differently to PCM depending on the pattern of the acquired immune response developed. The onset of protective immune response is notably mediated by neutrophils (PMN) that play an important role through directly killing the fungi and also by interacting with other cell types to modulate the acquired protective immune response that may follow. In that way, this study aimed to present and compare different experimental models of PCM (intraperitoneal and subcutaneous) regarding PMN production and maturation inside femoral bone marrow and also PMN infiltration in peritoneal and subcutaneous exudates of resistant and susceptible mice. We also assessed the fungal colony forming units and the levels of soluble inflammatory mediators (LTB4, KC, IFN-γ, GM-CSF, and IL-10) inside subcutaneous air-pouches to compare the efficiency of the PMN present at this site in relation to the two main neutrophil functions: initial lysis of the invading pathogen and modulation of the acquired immune response.P. brasiliensisinoculated intraperitoneally was able to disseminate to the bone marrow of susceptible mice, causing a more marked alteration of PMN production and maturation than that observed after resistant mice infection by the same route. Subcutaneous air-pouch inoculation ofP. brasiliensiselicited a controlled and limited infection that produced a PMN-rich exudate, thus favoring the study of the interaction between the fungus and the neutrophils. Susceptible mice produced higher numbers of PMN; however, these cells were less effective in killing the fungi. Inflammatory cytokines were more pronounced in resistant mice, which supports their PCM raised resistance. |
| Databáze: | OpenAIRE |
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