Self-Healable Porous Polyampholyte Hydrogels with Higher Water Content as Cell Culture Scaffolds for Tissue Engineering Applications

Autor: Jui Hsiang Liu, Chun Yen Liu, Kai Ti Chang, Chun Yu Kao, Yu Ting Lin, Yi Hua Hung
Rok vydání: 2020
Předmět:
Zdroj: ACS Applied Bio Materials. 3:5446-5453
ISSN: 2576-6422
DOI: 10.1021/acsabm.0c00757
Popis: In this paper, we first demonstrate the control of the film pore size using neutral hydrophilic 2-hydroxyethyl methacrylate (HEMA) content. To improve the mechanical properties of a polyampholyte (PA), both HEMA and the cross-linker N,N′-methylenebisacrylamide (Bis-Am) were introduced into the PA chain. The predesigned copolymers showed great mechanical properties and optical behavior. The introduction of HEMA significantly increased the water content of the polymer, leading to the formation of porous structures in xerogels. The dynamic interaction between the positive and negative termini of the PA endowed the hydrogels with self-healing ability. The synthesized chemically cross-linked PA gels showed high stability in saline solution. The biocompatibility of the PA gels was confirmed using a cytotoxicity test of cells attached to the synthesized PA-X-2 and PA/HEMA-90/10-X-0.5. The results of this investigation indicate that the synthesized PA gels are applicable as a polymeric scaffold for cell culture.
Databáze: OpenAIRE