Separation by high-performance liquid chromatography of α- and β-amino acids: Application to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase
| Autor: | Aberhart Dj |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Lysine
Biophysics Biochemistry High-performance liquid chromatography Serine Isomerism Animals Amino Acids Tyrosine Intramolecular Transferases Molecular Biology Chromatography High Pressure Liquid Amino Acid Isomerases chemistry.chemical_classification Chromatography Lysine 2 3-aminomutase Chemistry Rats Inbred Strains Cell Biology Rats Amino acid Aminoisobutyric acid Liver bacteria Leucine |
| Zdroj: | Analytical Biochemistry. 169:350-355 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(88)90294-1 |
| Popis: | Several pairs of alpha- and beta-amino acids were well separated as the ortho-phthalaldehyde derivatives by reversed phase HPLC. These included alpha- and beta-lysine, leucine, tyrosine, serine, aminoisobutyric acid, and beta-hydroxyvaline and its positional isomer. The separation was applicable to assays of lysine 2,3-aminomutase and leucine 2,3-aminomutase. However, for unknown reasons, no leucine 2,3-aminomutase activity could be found in rat liver homogenate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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