The relationship between adrenocortical function and Hsp70 expression in socially isolated Japanese quail
| Autor: | A. F. Soleimani, Idrus Zulkifli, A. R. Raha, Abdul Rahman Omar |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Physiology Coturnix Biology Biochemistry Basal (phylogenetics) chemistry.chemical_compound Corticosterone Internal medicine biology.animal medicine Animals HSP70 Heat-Shock Proteins Molecular Biology Metyrapone Adrenal cortex Myocardium Brain Feeding Behavior Ascorbic acid biology.organism_classification Quail Hsp70 Endocrinology medicine.anatomical_structure Social Isolation chemistry Adrenal Cortex medicine.drug |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 161:140-144 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/j.cbpa.2011.10.003 |
| Popis: | Physiological responses to social isolation stress were compared in 56-day-old male Japanese quail. Birds were fed pretreated diets for 3 days as follows: (i) Basal diet (control); (ii) Basal diet+1500 mg/kg metyrapone (BM); (iii) Basal diet+30 mg/kg corticosterone (BCO); (iv) Basal diet+250 mg/kg ascorbic acid (BC); (v) Basal diet+250 mg/kg α-tocopherol (BE); (vi) Basal diet+250 mg/kg ascorbic acid and 250 mg/kg α-tocopherol (BCE). The birds were subsequently socially isolated in individual opaque brown paper box for 2 hours. Plasma corticosterone (CORT) concentration and heart and brain heat shock protein 70 (Hsp 70) expressions were determined before stress and immediately after stress. Two hours of isolation stress elevated CORT concentration significantly in the control and BE birds but not in the BC, BCE and BM birds. There was a significant reduction in CORT concentration after isolation stress in the BCO group. Isolation stress increased Hsp 70 expression in the brain and heart of control and BM birds. However, brain and heart Hsp 70 expressions were not significantly altered in the isolated BC, BCE and BE birds. Although, the CORT concentration of BM birds was not affected by isolation stress, Hsp70 expression in both brain and heart were significantly increased. Moreover, exogenous corticosterone supplementation did not result in elevation of Hsp 70 expression. It can be concluded that, although Hsp 70 induction had not been directly affected by CORT concentration, it may be modulated by the HPA axis function via activation of ACTH. |
| Databáze: | OpenAIRE |
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