New genetic polymorphisms within ovine β- and αS2-caseins
| Autor: | Giulio Pagnacco, M Berbenni, Stefania Chessa, Mina Martini, G. Ceriotti, D. Rignanese, Anna Maria Caroli |
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| Rok vydání: | 2010 |
| Předmět: |
Silent mutation
Genetics alphaS2 -Casein education.field_of_study Ovine milk caseine latte genetica ovini Population Gene polymorphisms Intron Biology Molecular biology Food Animals Polymorphism (computer science) Casein Animal Science and Zoology education Transversion Ovine milk Gene polymorphisms Beta-Casein alphaS2 -Casein Beta-Casein Allele frequency Gene |
| Zdroj: | Small Ruminant Research. 88:84-88 |
| ISSN: | 0921-4488 |
| DOI: | 10.1016/j.smallrumres.2009.12.014 |
| Popis: | Casein genes in ruminants are organized in a cluster including α S1 -casein ( CSN1S1 ), β-casein ( CSN2 ), α S2 -casein ( CSN1S2 ), and κ-casein ( CSN3 ). Considering the results obtained in cattle and goat species concerning the influence of genetic polymorphisms on milk composition, quality, and technological properties, research on the polymorphisms of ewe's milk has known a new impulse in the last decade. A total of 54 samples belonging to the Massese dairy breed, to the double pourpose (milk and meat) Garfagnina population and to the Pomarancina and Zerasca meat populations, reared in the Centre of Italy, were analysed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). New PCR-SSCP patterns were found in both CSN2 and CSN1S2 genes. Sequencing of the samples carrying the new patterns revealed 2 new variants at CSN2 gene. Frequencies of the 2 variants in the samples analysed were 0.18 and 0.02. The less common variant is characterized by a silent mutation in the triplet coding for Gln 192 , whereas in the more frequent one a C to A transversion is responsible for the aminoacid exchange Leu 196 → Ile 196 . At the CSN1S2 gene only a new variant was found with a frequency of 0.02. The variant is characterized by two linked mutations: a C to G transversion, responsible for the aminoacid change Asn 200 → Lys 200 already described at the protein level, and a T to A transversion at the 14th nucleotide of the 16th intron. The ovine caseins deserve a bigger attention that has to be directed to a complete characterization of the described variants and to the understanding of their functional meaning. |
| Databáze: | OpenAIRE |
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