Skeletal Muscle Regenerative Potential of Human MuStem Cells following Transplantation into Injured Mice Muscle
| Autor: | Mireille Ledevin, Bruno Delorme, Laurence Dubreil, Lucie Chabrand, Gillian Butler-Browne, Armelle Magot, Yann Péréon, Sabrina Viau, Karl Rouger, Laëtitia Guével, Blandine Lieubeau, Florence Robriquet, Candice Babarit, Elisa Negroni, Vincent Mouly, Isabelle Leroux, Chantal Thorin, Antoine Hamel, Cindy Schleder, Charlotte Saury, Judith Lorant |
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| Přispěvatelé: | Physiopathologie Animale et bioThérapie du muscle et du système nerveux (PAnTher), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Immuno-Endocrinologie Cellulaire et Moléculaire (IECM), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes, Institut de Myologie, Université Pierre et Marie Curie - Paris 6 (UPMC), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS), Biotherapy division, MacoPharma Tourcoing, Chirurgie infantile, Centre hospitalier universitaire de Nantes (CHU Nantes), explorations fonctionnelles, centre de référence des maladies neuromusculaires, Laboratoire de Physiopathologie Animale et Pharmacologie Fonctionnelle, Ecole Nationale Vétérinaire Agroalimentaire et de l'Alimentation Nantes Atlantique (ONIRIS), Biotherapy Divison, Explorations fonctionnelles, centre de références des maladies neuromusculaires, 'Association Francaise contre les Myopathies' (AFM) [15990], FEDER (Fonds Europeens de Developpement Regional) [37085, 38436], French government [ANR-10-IBHU-005], Nantes Metropole, Pays de la Loire Region, ProdInra, Archive Ouverte, Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN)-Ecole Nationale Vétérinaire de Nantes-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Développement et Pathologie du Tissu Musculaire (DPTM), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Nantes, Université Pierre et Marie Curie (Paris 6) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
pseudohypertrophic childhood muscular dystrophy myopathie de duchenne Myoblasts Skeletal [SDV]Life Sciences [q-bio] cellule souche adulte Clinical uses of mesenchymal stem cells regenerative medicine Biology Muscle Development Stem cell marker Mice 03 medical and health sciences Drug Discovery DMD Genetics medicine Animals Humans Regeneration Progenitor cell skeletal muscle Muscle Skeletal Molecular Biology Cells Cultured Cell Proliferation Pharmacology Myogenesis Mesenchymal stem cell Skeletal muscle Cell Differentiation thérapie cellulaire Muscular Dystrophy Animal MuStem Cell biology Muscular Dystrophy Duchenne [SDV] Life Sciences [q-bio] Adult Stem Cells 030104 developmental biology medicine.anatomical_structure Immunology Molecular Medicine Original Article Stem cell cell therapy régénération musculaire Stem Cell Transplantation Adult stem cell human adult stem cells |
| Zdroj: | Molecular Therapy Molecular Therapy, Cell Press, 2018, 26 (2), ⟨10.1016/j.ymthe.2017.10.013⟩ Molecular Therapy, 2018, 26 (2), ⟨10.1016/j.ymthe.2017.10.013⟩ Molecular Therapy, Nature Publishing Group, 2017, ⟨10.1016/j.ymthe.2017.10.013⟩ |
| ISSN: | 1525-0016 1525-0024 |
| Popis: | After intra-arterial delivery in the dystrophic dog, allogeneic muscle-derived stem cells, termed MuStem cells, contribute to long-term stabilization of the clinical status and preservation of the muscle regenerative process. However, it remains unknown whether the human counterpart could be identified, considering recent demonstrations of divergent features between species for several somatic stem cells. Here, we report that MuStem cells reside in human skeletal muscle and display a long-term ability to proliferate, allowing generation of a clinically relevant amount of cells. Cultured human MuStem (hMuStem) cells do not express hematopoietic, endothelial, or myo-endothelial cell markers and reproducibly correspond to a population of early myogenic-committed progenitors with a perivascular/mesenchymal phenotypic signature, revealing a blood vessel wall origin. Importantly, they exhibit both myogenesis in vitro and skeletal muscle regeneration after intramuscular delivery into immunodeficient host mice. Together, our findings provide new insights supporting the notion that hMuStem cells could represent an interesting therapeutic candidate for dystrophic patients. |
| Databáze: | OpenAIRE |
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