Exposure to gemfibrozil and atorvastatin affects cholesterol metabolism and steroid production in zebrafish (Danio rerio)
| Autor: | Aziz A. Al-Habsi, Thomas W. Moon, Andrey Massarsky |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Physiology Atorvastatin medicine.medical_treatment Gene Expression Biology Ecotoxicology Biochemistry Steroid 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Gemfibrozil Animals RNA Messenger Molecular Biology Zebrafish Testosterone Triglycerides Cholesterol Reproduction Oxidoreductases N-Demethylating Metabolism Zebrafish Proteins biology.organism_classification Sterol regulatory element-binding protein Diet Protein Transport 030104 developmental biology Endocrinology chemistry Receptors LDL lipids (amino acids peptides and proteins) Female Hydroxymethylglutaryl CoA Reductases Steroids Aryl Hydrocarbon Hydroxylases Water Pollutants Chemical medicine.drug |
| Zdroj: | Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 199 |
| ISSN: | 1879-1107 |
| Popis: | The commonly used lipid-lowering pharmaceuticals gemfibrozil (GEM) and atorvastatin (ATV) are detected in the aquatic environment; however, their potential effects on non-target fish species are yet to be fully understood. This study examined the effects of GEM and/or ATV on female and male adult zebrafish after a 30d dietary exposure. The exposure led to changes in several biochemical parameters, including reduction in cholesterol, triglycerides, cortisol, testosterone, and estradiol. Changes in cholesterol and triglycerides were also associated with changes in transcript levels of key genes involved with cholesterol and lipid regulation, including SREBP2, HMGCR1, PPARα, and SREBP1. We also noted higher CYP3A65 and atrogin1 mRNA levels in drug-treated male fish. Sex differences were apparent in some of the examined parameters at both biochemical and molecular levels. This study supports these drugs affecting cholesterol metabolism and steroid production in adult zebrafish. We conclude that the reduction in cortisol may impair the ability of these fish to mount a suitable stress response, whereas the reduction of sex steroids may negatively affect reproduction. |
| Databáze: | OpenAIRE |
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