Redondovirus DNA in human respiratory samples
| Autor: | Chiara Biagini, Lisa Macera, Paola Mazzetti, Michele Lai, Mauro Pistello, Ilaria Sciandra, M. Curcio, Ombretta Turriziani, Guido Antonelli, Pietro Giorgio Spezia, Fabrizio Maggi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine 030106 microbiology Respiratory tract Genome Viral Biology Genome Article Virus Pathogenesis Human redondovirus Feces 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine ReDoV DNA Virology Prevalence medicine Humans Sequencing Real-time PCR 030212 general & internal medicine Respiratory Tract Infections Gene Phylogeny Aged Retrospective Studies fungi DNA Viruses food and beverages Genetic Variation Sequence Analysis DNA Middle Aged DNA Virus Infections Infectious Diseases medicine.anatomical_structure Real-time polymerase chain reaction Italy Capsid chemistry DNA Viral Capsid Proteins Female DNA |
| Zdroj: | Journal of Clinical Virology |
| Popis: | Highlights • ReDoV infection may not be systemic or the presence of circulating virus can be only intermittent. • ReDoV infection is mainly present in the respiratory tract. Background Redondovirus (ReDoV) is a recently discovered circular, Rep-encoding single-stranded DNA (CRESS-DNA) virus in humans. Its pathogenesis and clinical associations are still completely unknown. Methods The presence of ReDoV DNA was investigated in biological specimens of 543 Italian subjects by in-house developed PCR assays. Results The overall ReDoV prevalence was about 4% (23 of 543 samples). The virus was detected in 22 of 209 (11 %) respiratory samples. One stool sample was also ReDoV positive. Viral DNA was not found in blood samples from immunocompetent and immunosuppressed subjects and cerebrospinal fluids from patients with neurological diseases. Genomic nucleotide differences were detected among the ReDoV isolates by sequencing a 582-nucleotide fragment of the capsid gene of the viral genome. Conclusions The results demonstrate that ReDoV is mainly present in the respiratory tract of infected people. Further investigations are needed to reveal possible clinical implications of this new CRESS-DNA virus in humans. |
| Databáze: | OpenAIRE |
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