Analysis of Phenolic Compounds and Antioxidant Activity in Wild Blackberry Fruits

Autor: Jan Oszmiański, Tomasz Cebulak, Mirosława Teleszko, Krzysztof Oklejewicz, Paulina Nowicka, Aneta Wojdyło
Rok vydání: 2015
Předmět:
Zdroj: International Journal of Molecular Sciences, Vol 16, Iss 7, Pp 14540-14553 (2015)
International Journal of Molecular Sciences
Volume 16
Issue 7
Pages 14540-14553
ISSN: 1422-0067
DOI: 10.3390/ijms160714540
Popis: Twenty three different wild blackberry fruit samples were assessed regarding their phenolic profiles and contents (by LC/MS quadrupole time-of-flight (QTOF) and antioxidant activity (ferric reducing ability of plasma (FRAP) and 2,2-azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS)) by two different extraction methods. Thirty four phenolic compounds were detected (8 anthocyanins, 15 flavonols, 3 hydroxycinnamic acids, 6 ellagic acid derivatives and 2 flavones). In samples, where pressurized liquid extraction (PLE) was used for extraction, a greater increase in yields of phenolic compounds was observed, especially in ellagic acid derivatives (max. 59%), flavonols (max. 44%) and anthocyanins (max. 29%), than after extraction by the ultrasonic technique extraction (UAE) method. The content of phenolic compounds was significantly correlated with the antioxidant activity of the analyzed samples. Principal component analysis (PCA) revealed that the PLE method was more suitable for the quantitative extraction of flavonols, while the UAE method was for hydroxycinnamic acids.
Databáze: OpenAIRE