Peptide attachment to extremities of liposomal surface grafted PEG chains: preparation of the long-circulating form of laminin pentapeptide, YIGSR
| Autor: | Bhagya Puntambekar, Parthena Boulikas, Samuel Zalipsky, Martin C. Woodle, Charles Engbers |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Metabolic Clearance Rate Molecular Sequence Data Biomedical Engineering Pharmaceutical Science Bioengineering Peptide Pentapeptide repeat Polyethylene Glycols Rats Sprague-Dawley chemistry.chemical_compound PEG ratio Animals Tissue Distribution Amino Acid Sequence Pharmacology chemistry.chemical_classification Drug Carriers Liposome Chemistry Phosphatidylethanolamines Vesicle Organic Chemistry Rats Biochemistry Liposomes Laminin Drug carrier Oligopeptides Ethylene glycol Biotechnology Conjugate |
| Zdroj: | Bioconjugate Chemistry. 6:705-708 |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/bc00036a008 |
| Popis: | Poly(ethylene glycol) (PEG)-grafted liposomes offer new opportunities as long-circulating platforms presenting biologically relevant ligands. In pursuit of this goal, liposomal conjugates of YIGSR were prepared by mild periodate oxidation of TYIGSR-NH2 and incubation of the product with hydrazide-PEG-(distearoylphosphatidyl)ethanolamine-containing liposomes. The peptide-carrying liposomes, with up to 500 YIGSR residues per vesicle, despite exhibiting faster blood clearance rates than the parent liposomes in rats, remained in circulation for extended periods of time. Mean residence times for the parent liposomal formulation and conjugated preparations containing 200 and 500 YIGSR residues per vesicle were 28, 25, and 23 h, respectively. The results have important implications for systemic delivery of peptides and for their use as targeting moieties for PEG-grafted liposomes. |
| Databáze: | OpenAIRE |
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