Polypeptide fluoroacetate derivatives Synthesis and 19F n.m.r
| Autor: | Carter B. Green, Michael E. Mount, David Y. Jackson, Mark J. Kurth |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Magnetic Resonance Spectroscopy biology Chemistry Stereochemistry Fluoroacetates Serum albumin Fluorine Sulfonic acid Biochemistry Chemical synthesis Mass Spectrometry chemistry.chemical_compound Succinimide Amide biology.protein Fluoroacetate Indicators and Reagents Amine gas treating Bovine serum albumin Peptides Haptens |
| Zdroj: | International Journal of Peptide and Protein Research. 31:388-395 |
| ISSN: | 0367-8377 |
| DOI: | 10.1111/j.1399-3011.1988.tb00047.x |
| Popis: | The synthesis and characterization of monofluoroacetyl (MFAc) functionalized haptens are described. These were covalently bound to polypeptide carriers (bovine serum albumin and poly-D-lysine) by primary amine/succinimide ester or primary amine/acid chloride coupling. Epitopic densities of the resulting antigens were determined by both 19F n.m.r. and picryl sulfonic acid assays. 19F n.m.r. experiments defining the stability of MFAc ester and amide linkages as a function of media (including in vitro), time, and temperature are presented. These results indicate that MFAc functionalized antigens are well suited for further immunologic studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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