Transient receptor potential A1 increase glutamate release on brain stem neurons
| Autor: | Boram Sun, Sung-Il Bang, Young-Ho Jin |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Ankyrins
Male Agonist Patch-Clamp Techniques medicine.drug_class Glutamic Acid Tetrodotoxin Rats Sprague-Dawley Transient receptor potential channel chemistry.chemical_compound Isothiocyanates Solitary Nucleus medicine Autonomic reflex Animals TRPA1 Cation Channel Cells Cultured TRPC Cation Channels Neurons Chemistry General Neuroscience Solitary nucleus Glutamate receptor Excitatory Postsynaptic Potentials food and beverages Calcium Channel Blockers Ruthenium Red Rats Calcium Channel Agonists Electrophysiology medicine.anatomical_structure Capsaicin Biophysics Calcium Channels Neuron Neuroscience psychological phenomena and processes Cadmium |
| Zdroj: | NeuroReport. 20:1002-1006 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/wnr.0b013e32832d2219 |
| Popis: | Low temperature and noxious chemicals activate transient receptor potential (TRP) A1 channel in spinal nociceptive neurons. TRPA1 ligands are found as common ingredients of pungent spices and can trigger autonomic reflexes and alter blood pressure. Recent discovery of TRPA1 on visceral afferent pathway suggests such nonnociceptive responses may be closely related to TRPA1. To understand potential contributions of TRPA1 on autonomic response, we have tested TRPA1 agonist allyl isothiocyanate (AITC) effects on mechanically dispersed nucleus tractus solitarii neurons. AITC of 200 microM increased glutamate release in 58% of tested neurons and TRP channel antagonist ruthenium red (50 microM) blocked the AITC actions. tetrodotoxin and Cd did not eliminate AITC-evoked glutamate release increase. TRPA1 expressed in capsaicin-sensitive and insensitive neurons. |
| Databáze: | OpenAIRE |
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