Effects of 17beta-estradiol exposure in the mussel Mytilus galloprovincialis
| Autor: | Cinta Porte, Gemma Janer, Rémi Thibaut, Ramon Lavado |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
P450-aromatase Aquatic Science Oceanography Sex hormone-binding globulin Aromatase Internal medicine Microsomes medicine Animals Testosterone Gonads chemistry.chemical_classification biology Esterification Estradiol Palmitoyl Coenzyme A Histological Techniques Fatty acid Esters General Medicine Mussel biology.organism_classification Bivalvia Pollution Mytilus Endocrinology chemistry Mytilus galloprovincialis Gene Expression Regulation Acyltransferase biology.protein Acyl Coenzyme A Hormone |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0141-1136 |
| Popis: | 4 pages, 2 figures.-- PMID: 15178065 [PubMed].-- Printed version published in issue Aug-Dec 2004.-- Issue title: "Twelfth International Symposium on Pollutant Responses in Marine Organisms" (Tampa Bay, FL, United States, May 9-13, 2003). Mussels Mytilus galloprovincialis were exposed to different concentrations of estradiol (20, 200, and 2000 ng/l) in a semi-static regime (1-day dosing intervals) for up to 7 days in an attempt to see how mussels dealt with exogenous estrogenic compounds. Sex hormone levels were determined in whole tissue. Free-estradiol was only significantly elevated at the highest exposure dose (up to 10-fold). Most of the estradiol was in the tissues as fatty acid esters (>78%), which sharply increased in a dose-dependent manner (from 4 ng/g in controls to 258 ng/g at the high exposure group). In contrast, neither free nor esterified testosterone levels showed significant differences between control and exposure groups. The results suggest the existence of mechanisms that allow mussels to maintain their hormonal status, and the important role that fatty acid esterification may play within those mechanisms. Synthesis and conjugation rates of estradiol were further investigated by measuring the activity of P450 aromatase, and palmitoyl-CoA:estradiol acyltransferase, in digestive gland microsomal fractions. Overall, the study contributes to the better knowledge of molluscan endocrinology, and defines new mechanisms of regulation of free steroid-levels in mussels. Predoctoral fellowship from the Spanish Government and partial support from EU Project BEEP (ENVK3-CT-2000-00025). |
| Databáze: | OpenAIRE |
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