Plant regeneration through somatic embryogenesis in the forage grass Caucasian bluestem (Bothriochloa caucasica)

Autor:	C. I. Franklin, Tony N. Trieu, R. A. Gonzales
Rok vydání:	1990
Předmět:	Somatic embryogenesis Bothriochloa food and beverages Kanamycin Plant Science General Medicine Biology biology.organism_classification Acclimatization Micropropagation Callus Botany Shoot medicine Agronomy and Crop Science Explant culture medicine.drug
Zdroj:	Plant Cell Reports. 9
ISSN:	1432-203X 0721-7714
DOI:	10.1007/bf00232269
Popis:	Plantlets were regenerated from cultured seed explants of the forage grass Caucasian bluestem [Bothriochloa caucasica (Trin.) C.E. Hubbard] via somatic embryogenesis. Embryogenic callus was produced in four weeks when surface sterilized seeds were cultured on a medium containing MS-salts, B-5 vitamins, 12 mM L-proline, 2% sucrose, 0.8% agar and 5μM 2,4-D. Plantlets were regenerated in 6-8 weeks after culture initiation. Healthy root and shoot systems were produced within three weeks after the plantlets were transferred to a medium lacking 2,4-D. Approximately 95% of the plantlets survived greenhouse acclimation and produced healthy plants and viable seeds. Caucasian bluestem callus cultures exhibit natural resistance to kanamycin. High levels of kanamycin (up to 800 mg/l) did not completely inhibit callus growth. However, the regeneration of healthy-plantlets was completely inhibited by kanamycin even at low levels (50 mg/l).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dc5792a02e97c52d0e8bc73a846e0d8 https://doi.org/10.1007/bf00232269 Zobrazit plný text záznamu Full text from SpringerLink