Frequencies of Immune Cells in the Human Small Bowel During Normal Gestation and in Necrotizing Enterocolitis

Autor:	Niels Qvist, Sönke Detlefsen, Lene Rasmussen, Tine Plato Hansen, Steffen Husby, Paul Peterslund
Rok vydání:	2019
Předmět:	0301 basic medicine Male Pathology medicine.medical_specialty T cell Tryptase Gestational Age 030105 genetics & heredity Infant Newborn Diseases Pathology and Forensic Medicine 03 medical and health sciences immune cells normal small bowel 0302 clinical medicine Immune system Enterocolitis Necrotizing Risk Factors Submucosa Intestine Small medicine Humans Intestinal Mucosa gestational age B cell Histiocyte necrotizing enterocolitis 030219 obstetrics & reproductive medicine biology business.industry Infant Newborn General Medicine Mast cell medicine.disease Fetal Diseases medicine.anatomical_structure Pediatrics Perinatology and Child Health Necrotizing enterocolitis biology.protein Female business
Zdroj:	Peterslund, P, Rasmussen, L, Qvist, N, Hansen, T P, Husby, S & Detlefsen, S 2019, ' Frequencies of Immune Cells in the Human Small Bowel During Normal Gestation and in Necrotizing Enterocolitis ', Fetal and Pediatric Pathology, vol. 38, no. 2, pp. 153-166 . https://doi.org/10.1080/15513815.2018.1561774
ISSN:	1551-3823
DOI:	10.1080/15513815.2018.1561774
Popis:	BACKGROUND: Only few studies have quantitated the frequencies of immune cells in the small bowel mucosa and submucosa during gestation. The aims of this study were to describe the frequencies of T and B cells, eosinophils and mast cells in the normal small bowel mucosa and submucosa (NSB) in relation to gestational age (GA) and in the uninvolved small bowel (USB) of premature newborns with necrotizing enterocolitis (NEC).METHODS: We obtained 36 NSB specimens (GA 12-41 weeks) and 8 NEC-USB specimens (GA 24-32 weeks) from autopsies and surgeries and performed immunostaining for CD3, CD79a, BMK-13 and tryptase as well as the histochemical stains giemsa and toluidine blue. Qualitative histological evaluation and two different quantitative cell-samplings were performed using digital imaging analysis with both TissuemorphDP® and newCAST® software. Linear regression analysis was performed with cell frequency as the dependent variable and GA and USB as the independent variables.RESULTS: In the NSB specimens, we found significant linear correlations between cell frequencies and GA for all examined cell types, though B cell frequencies reached a plateau midway through gestation. In the USB cases, submucosal mast cell frequencies were higher than in the NSB specimens, while T cell frequencies were lower. In USB of NEC patients, we found a significant increase of mast cells and a significant decrease of T cells compared to NSB.CONCLUSION: Throughout gestation, we found an increase of all examined immune cell types in the normal small bowel, while the number of B cells came to a standstill at midway. Future studies should examine subtypes of T cells and also include histiocytes. A larger amount of small bowel specimens, covering the full gestational age, would be of great value.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dc50e7d34255872ab2d90db2fea8612 https://pubmed.ncbi.nlm.nih.gov/30689475 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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