Comparative study of seeding methods for three-dimensional polymeric scaffolds

Autor: C. R. Culberson, W. D. Holder, W. D. Roland, David J. Mooney, K. G. Greene, R. J. Beiler, Petra Eiselt, Karen J. L. Burg, A. B. Loebsack, Craig Halberstadt
Rok vydání: 2000
Předmět:
Zdroj: Journal of Biomedical Materials Research. 52:576-576
ISSN: 1097-4636
0021-9304
DOI: 10.1002/1097-4636(20001205)52:3<576::aid-jbm18>3.0.co;2-c
Popis: Development of tissue-engineered devices may be enhanced by combining cells with porous absorbable polymeric scaffolds before implantation. The cells are seeded throughout the scaffolds and allowed to proliferate in vitro for a predetermined amount of time. The distribution of cells throughout the porous material is one critical component determining success or failure of the tissue-engineered device. This can influence both the successful integration of the device with the host tissue as well as the development of a vascularized network throughout the entire scaffold volume. This research sought to compare different seeding and proliferation methods to select an ideal method for a polyglycolide/aortic endothelial cell system. Two seeding environments, static and dynamic, and three proliferation environments, static, dynamic, and bioreactor, were analyzed, for a total of six possible methods. The six seeding and proliferation combinations were analyzed following a 1-week total culture time. It was determined that for this specific system, dynamic seeding followed by a dynamic proliferation phase is the least promising method and dynamic seeding followed by a bioreactor proliferation phase is the most promising.
Databáze: OpenAIRE
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