Nasopharyngeal Microbiota Analysis in Healthy and Otitis-prone Children
| Autor: | Paola Marchisio, Miriam Fattizzo, Stefano Aliberti, Sara Torretta, Michele Gaffuri, Francesco Folino, Francesco Blasi, Martina Oriano, Luca Ruggiero |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Microbiology (medical) food.ingredient Corynebacterium medicine.disease_cause Microbiology Pathogenesis 03 medical and health sciences 0302 clinical medicine food Nasopharynx 030225 pediatrics Haemophilus medicine Humans 030212 general & internal medicine Moraxella Bacteria Tympanic Membrane Perforation biology Streptococcus business.industry Microbiota Bacterial Infections biology.organism_classification Otitis Media Cross-Sectional Studies Infectious Diseases Otitis medicine.anatomical_structure Dolosigranulum Child Preschool Carrier State Pediatrics Perinatology and Child Health Female Disease Susceptibility medicine.symptom business Respiratory tract |
| Zdroj: | Pediatric Infectious Disease Journal. 40:16-21 |
| ISSN: | 0891-3668 |
| DOI: | 10.1097/inf.0000000000002895 |
| Popis: | Background Recurrent acute otitis media (RAOM) is common in children, and it may result in spontaneous tympanic membrane perforation (STMP), management of which is often challenging. In the upper respiratory tract (URT), resident microorganisms play a pivotal role in otitis media pathogenesis and prevention, as they are able to inhibit the colonization process and otopathogens growth. In particular, Dolosigranulum spp. and Corynebacterium spp. have been associated with respiratory health in several studies. This study aims at comparing both nasopharyngeal microbiota of children with RAOM versus matched controls and nasopharyngeal microbiota of children with a history of RAOM with STMP. Method Nasopharyngeal swabs were collected from 132 children, median age 3.51 (2.13-4.72), including 36 healthy children, 50 with RAOM without STMP, and 46 with RAOM with STMP. Bacterial DNA was subsequently extracted and 16S rRNA gene V3-V4 regions were polymerase chain reaction amplified and sequenced using Illumina MiSeq technology. Results A higher relative abundance of Dolosigranulum and Corynebacterium genera was detected in the nasopharynx of healthy children (16.5% and 9.3%, respectively) in comparison with RAOM without STMP (8.9% and 4.3%, respectively) and RAOM with STMP (5.2% and 2.8%, respectively). A decreasing pattern in relative abundance of these 2 pivotal genera through disease severity was detected. In all groups, the most abundant genera were Moraxella, Streptococcus and Haemophilus, followed by Dolosigranulum and Corynebacterium. Conclusions Our study provides a characterization of the URT microbiota in otitis-prone children with and without history of recurrent STMP, suggesting that the role of Dolosigranulum and Corynebacterium in regulating the healthy URT microbiota should be further studied. |
| Databáze: | OpenAIRE |
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