Functional imaging of brain organoids using high-density microelectrode arrays

Autor: Manuel Schröter, Congwei Wang, Marco Terrigno, Philipp Hornauer, Ziqiang Huang, Ravi Jagasia, Andreas Hierlemann
Rok vydání: 2022
Předmět:
Zdroj: MRS Bulletin
MRS Bulletin, 47 (6)
ISSN: 0883-7694
1938-1425
DOI: 10.1557/s43577-022-00282-w
Popis: Studies have provided evidence that human cerebral organoids (hCOs) recapitulate fundamental milestones of early brain development, but many important questions regarding their functionality and electrophysiological properties persist. Highdensity microelectrode arrays (HD-MEAs) represent an attractive analysis platform to perform functional studies of neuronal networks at the cellular and network scale. Here, we use HD-MEAs to derive large-scale electrophysiological recordings from sliced hCOs. We record the activity of hCO slices over several weeks and probe observed neuronal dynamics pharmacologically. Moreover, we present results on how the obtained recordings can be spike-sorted and subsequently studied across scales. For example, we show how to track single neurons across several days on the HD-MEA and how to infer axonal action potential velocities. We also infer putative functional connectivity from hCO recordings. The introduced methodology will contribute to a better understanding of developing neuronal networks in brain organoids and provide new means for their functional characterization.
MRS Bulletin, 47 (6)
ISSN:0883-7694
ISSN:1938-1425
Databáze: OpenAIRE
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