Effects of some drugs on human cord blood erythrocyte carbonic anhydrases I and II: an in vitro study
| Autor: | T. Abdulkadir Coban, Mehmet Aktas, Murat Cankaya, Mehmet Kuzucu, İlhami Gül |
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| Přispěvatelé: | EBYÜ, Tıp Fakültesi |
| Rok vydání: | 2011 |
| Předmět: |
Carbonic Anhydrase I
Erythrocytes Carbonic anhydrase II education Pharmacology In Vitro Techniques Carbonic Anhydrase II Chromatography Affinity Ranitidine Carbonic anhydrase Drug Discovery Ceftizoxime medicine In vitro study Humans Carbonic Anhydrase Inhibitors biology Chemistry General Medicine Fetal Blood Biochemistry Cord blood Ceftriaxone biology.protein cord blood Electrophoresis Polyacrylamide Gel medicine.drug |
| Zdroj: | Journal of enzyme inhibition and medicinal chemistry. 27(5) |
| ISSN: | 1475-6374 |
| Popis: | In this [corrected] study, we purified hcbCA I and II from human cord blood erythrocytes using [corrected] Sepharose-4B-l [corrected] tyrosine-sulfanilamide affinity gel chromatography. [corrected]. The inhibition effects of ampicillin sulfate, ceftriaxone, ceftizoxime and ranitidine on hcbCA I and hcbCA II were also monitored. [corrected]. IC(50) values for ceftriaxone, ceftizoxime and ranitidine were found to be 27.l, 79.4 and 55.5 µM, respectively, [corrected] for hcbCA I, and [corrected] 21.0, 79.1 and 66.1 µM, respectively, [corrected] for hcbCA II. [corrected]. According to these results, ampicillin [corrected] sulfate inhibited only hcbCAII and IC(50) value [corrected] of this antibiotic was found to be 56.8 µM. All [corrected] substances were found to be [corrected] non-competitive inhibitors. It is important to study the inhibition effects of these drugs on hcbCA I and II izoenzymes as pregnant women are often prescribed these antibiotics. [corrected]. For this reason, the dosage of [corrected] these drugs should be carefully evaluated [corrected] to minimize side effects. |
| Databáze: | OpenAIRE |
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