Differential regulation of grain sucrose accumulation and metabolism in Coffea arabica (Arabica) and Coffea canephora (Robusta) revealed through gene expression and enzyme activity analysis
| Autor: | Magali Eychenne, Chenwei Lin, Anneke Prins, Victoria Caillet, Steve Tanksley, Séverine Foucrier, Isabelle Privat, Thibaut Epalle, James Gerard Mccarthy, Laurianne Kandalaft, Christine H. Foyer |
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| Rok vydání: | 2008 |
| Předmět: |
Sucrose
DNA Complementary Physiology Coffea Plant Science Fructose Coffea canephora Genes Plant Polymerase Chain Reaction chemistry.chemical_compound Gene Expression Regulation Plant Botany Coffee bean biology beta-Fructofuranosidase Coffea arabica fungi food and beverages biology.organism_classification Plant Leaves Invertase Glucose chemistry Vacuoles biology.protein Sucrose synthase Sucrose-phosphate synthase Edible Grain |
| Zdroj: | The New phytologistReferences. 178(4) |
| ISSN: | 1469-8137 |
| Popis: | * Coffea arabica (Arabica) and Coffea canephora (Robusta) are the two main cultivated species used for coffee bean production. Arabica genotypes generally produce a higher coffee quality than Robusta genotypes. Understanding the genetic basis for sucrose accumulation during coffee grain maturation is an important goal because sucrose is an important coffee flavor precursor. * Nine new Coffea genes encoding sucrose metabolism enzymes have been identified: sucrose phosphate synthase (CcSPS1, CcSPS2), sucrose phosphate phosphatase (CcSP1), cytoplasmic (CaInv3) and cell wall (CcInv4) invertases and four invertase inhibitors (CcInvI1, 2, 3, 4). * Activities and mRNA abundance of the sucrose metabolism enzymes were compared at different developmental stages in Arabica and Robusta grains, characterized by different sucrose contents in mature grain. * It is concluded that Robusta accumulates less sucrose than Arabica for two reasons: Robusta has higher sucrose synthase and acid invertase activities early in grain development - the expression of CcSS1 and CcInv2 appears to be crucial at this stage and Robusta has a lower SPS activity and low CcSPS1 expression at the final stages of grain development and hence has less capacity for sucrose re-synthesis. Regulation of vacuolar invertase CcInv2 activity by invertase inhibitors CcInvI2 and/or CcInvI3 during Arabica grain development is considered. |
| Databáze: | OpenAIRE |
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