Extracellular-like matrices and leukaemia inhibitory factor for in vitro culture of human primordial follicles
| Autor: | Ronit Abir, Galit Lerer-Serfaty, Benjamin Fisch, Michal Shachar-Goldenberg, Muayad A. Zahalka, Avi Ben-Haroush, Tzippy Shochat, Assiel J Younis, Dana Stav, Bethsabee Sabbah, Gania Kessler-Icekson |
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| Rok vydání: | 2017 |
| Předmět: |
Anti-Mullerian Hormone
0301 basic medicine endocrine system medicine.medical_specialty Cell Culture Techniques Reproductive technology Biology Leukemia Inhibitory Factor Andrology Extracellular matrix 03 medical and health sciences 0302 clinical medicine Endocrinology Ovarian Follicle Internal medicine Follicular phase Genetics medicine Extracellular Humans Molecular Biology 030219 obstetrics & reproductive medicine Estradiol Fertility Preservation In vitro Culture Media Extracellular Matrix 030104 developmental biology Reproductive Medicine biology.protein Immunohistochemistry Female Animal Science and Zoology Vitronectin Leukemia inhibitory factor Developmental Biology Biotechnology |
| Zdroj: | Reproduction, Fertility and Development. 29:1982 |
| ISSN: | 1031-3613 |
| Popis: | The possibility of maturing human primordial follicles in vitro would assist fertility restoration without the danger of reseeding malignancies. Leukaemia inhibitory factor (LIF) and certain culture matrices may promote human follicular growth. The present study compared human primordial follicular growth on novel culture matrices, namely human recombinant vitronectin (hrVit), small intestine submucosa (SIS), alginate scaffolds and human recombinant virgin collagen bioengineered in tobacco plant lines (CollPlant). The frozen–thawed ovarian samples that were used had been obtained from girls or young women undergoing fertility preservation. In the first part of the study, 20 samples were cultured for 6 days on hrVit or SIS with basic culture medium alone or supplemented with one of two concentrations of LIF (10 ng mL–1 and 100 ng mL–1), with and without LIF-neutralising antibody. In the second part of the study, 15 samples were cultured for 6 days on alginate scaffolds or CollPlant matrices with basic culture medium. Follicular development was assessed by follicular counts and classification, Ki67 immunohistochemistry and 17β-oestradiol and anti-Müllerian hormone measurements in spent media samples. Primordial follicular growth was not enhanced by LIF. Despite some significant differences among the four matrices, none appeared to have a clear advantage, apart from significantly more Ki67-stained follicles on alginate and CollPlant matrices. Further studies of other culture matrices and medium supplements are needed to obtain an optimal system. |
| Databáze: | OpenAIRE |
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