Transcriptome analysis of nitrogen-efficient rice over-expressing alanine aminotransferase

Autor:	Rebecka T. Carroll, Allen G. Good, Perrin H. Beatty, Tong Zhu, Ashok K. Shrawat
Rok vydání:	2009
Předmět:	Nitrogen Transgene Plant Science Genetically modified crops Biology Genes Plant Plant Roots Transcriptome Gene Expression Regulation Plant Gene expression Transgenes Gene Oligonucleotide Array Sequence Analysis Plant Proteins Oryza sativa Gene Expression Profiling fungi food and beverages Alanine Transaminase Oryza Plants Genetically Modified Gene expression profiling Acireductone dioxygenase Biochemistry RNA Plant Agronomy and Crop Science Plant Shoots Biotechnology
Zdroj:	Plant biotechnology journal. 7(6)
ISSN:	1467-7652
Popis:	Crop plants require nitrogen for key macromolecules, such as DNA, proteins and metabolites, yet they are generally inefficient at acquiring nitrogen from the soil. Crop producers compensate for this low nitrogen utilization efficiency by applying nitrogen fertilizers. However, much of this nitrogen is unavailable to the plants as a result of microbial uptake and environmental loss of nitrogen, causing air, water and soil pollution. We engineered rice over-expressing alanine aminotransferase (AlaAT) under the control of a tissue-specific promoter that showed a strong nitrogen use efficiency phenotype. In this study, we examined the transcriptome response in roots and shoots to the over-expression of AlaAT to provide insights into the nitrogen-use-efficient phenotype of these plants. Transgenic and control rice plants were grown hydroponically and the root and shoot gene expression profiles were analysed using Affymetrix Rice GeneChip microarrays. Transcriptome analysis revealed that there was little impact on the transgenic transcriptome compared with controls, with 0.11% and 0.07% differentially regulated genes in roots and shoots, respectively. The most up-regulated transcripts, a glycine-rich cell wall (GRP) gene and a gene encoding a hypothetical protein (Os8823), were expressed in roots. Another transgenic root-specific up-regulated gene was leucine rich repeat (LRR). Genes induced in the transgenic shoots included GRP, LRR, acireductone dioxygenase (OsARD), SNF2 ATP-translocase and a putative leucine zipper transcription factor. This study provides a genome-wide view of the response to AlaAT over-expression, and elucidates some of the genes that may play a role in the nitrogen-use-efficient phenotype.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1dbbe13b43a0125369c3ddd4a272c88c https://pubmed.ncbi.nlm.nih.gov/19508275 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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