Apigenin induces apoptosis in human lung cancer H460 cells through caspase- and mitochondria-dependent pathways
| Autor: | Siu Wan Ip, Ming Sung Yang, Jene John Fu, Chia Yu Ma, Yu Jie Chie, Kung Wen Lu, Te Chun Hsia, Hsu Feng Lu, Jing Gung Chung, Jai Sing Yang, Hung-Yi Chen |
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| Rok vydání: | 2010 |
| Předmět: |
Lung Neoplasms
Cell Survival DNA damage Health Toxicology and Mutagenesis Apoptosis Mitochondrion Toxicology chemistry.chemical_compound Cell Line Tumor Anticarcinogenic Agents Humans Apigenin Endoplasmic Reticulum Chaperone BiP Caspase Membrane Potential Mitochondrial chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug biology Caspase 3 Cytochrome c Cell Cycle General Medicine Chromatin Up-Regulation respiratory tract diseases Cell biology chemistry Cell culture biology.protein Calcium Apoptosis Regulatory Proteins Reactive Oxygen Species Signal Transduction |
| Zdroj: | Human & Experimental Toxicology. 30:1053-1061 |
| ISSN: | 1477-0903 0960-3271 |
| Popis: | Apigenin (4,5,7-trihydroxyflavone), a promising chemopreventive agent presented in fruits and vegetables, has been shown to induce cell cycle arrest and apoptosis in many types of human cancer cell lines. However, there is no available information to address the effects of apigenin on human lung cancer H460 cells. In the present studies, H460 cells were treated with apigenin for different time and then were analyzed for the morphological changes, induction of apoptosis, protein levels associated with apoptosis and results in dose-dependent induction of morphological changes, decrease in the percentage of viability, induced DNA damage and apoptosis; down-modulation of the protein expression of Bid, Bcl-2, procaspase-8; up-regulation of protein levels of Bax, caspase-3, AIF, cytochrome c, GRP78 and GADD153; decreased the levels of mitochondrial membrane potential and increased the productions of reactive oxygen species (ROS) and Ca2+ in H460 cells. Taken together, this is the first systematic in vitro study showing the involvement of apoptosis regulatory proteins as potential molecular targets of apigenin in human lung cancer H460 cells. |
| Databáze: | OpenAIRE |
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