Applicability of UV resistant Bacillus pumilus endospores as a human adenovirus surrogate for evaluating the effectiveness of virus inactivation in low-pressure UV treatment systems
| Autor: | Samuel L. Hayes, Jonathan Popovici, Jongseong Ryu, Jennifer L. Cashdollar, Laura A. Boczek, Mark R. Rodgers, Mano Sivaganesan, Hodon Ryu, Eric R. Rhodes, Jill Hoelle |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Virus inactivation Hot Temperature Ultraviolet Rays Bacillus Portable water purification 010501 environmental sciences medicine.disease_cause 01 natural sciences Microbiology Endospore Radiation Tolerance Cell Line Water Purification 03 medical and health sciences medicine Escherichia coli Humans Molecular Biology 0105 earth and related environmental sciences Levivirus Spores Bacterial biology Strain (chemistry) Bacillus pumilus Adenoviruses Human fungi Dose-Response Relationship Radiation biology.organism_classification Disinfection 030104 developmental biology Virus Inactivation Water Microbiology Uv treatment |
| Zdroj: | Journal of microbiological methods. 122 |
| ISSN: | 1872-8359 |
| Popis: | Recent studies have demonstrated the potential to use Bacillus pumilus endospores as a surrogate for human adenovirus (HAdV) in UV disinfection studies. The use of endospores has been limited by observations of batch-to-batch variation in UV sensitivity. This study reports on a propagation method that utilizes a commercially available medium to produce UV tolerant B. pumilus endospores with a consistent UV sensitivity. It is further demonstrated that the endospores of B. pumilus strain (ATCC 27142), produced using this protocol (half strength Columbia broth, 5 days incubation, with 0.1mM MnSO4), display a UV dose-response that is similar to that of HAdV. Endospore stocks could be stored in ethanol for up to two months at 4 °C without a significant change in UV sensitivity. Synergistic endospore damage was observed by pre-heat treatment of water samples followed by UV irradiation. UV tolerant B. pumilus endospores are a potential surrogate of HAdV for UV treatment performance tests in water utilities which do not have in-house research virology laboratories. |
| Databáze: | OpenAIRE |
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