Effects of growth factors on cell proliferation and matrix synthesis of low-density, primary bovine chondrocytes cultured in collagen I gels
| Autor: | Kanda Chaipinyo, Barry W Oakes, Marie-Paule Van Damme |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cell Survival
Cell Fluorescent Antibody Technique Chondrocyte law.invention Chondrocytes law Transforming Growth Factor beta medicine Articular cartilage repair Protein biosynthesis Animals Orthopedics and Sports Medicine Insulin-Like Growth Factor I Growth Substances Mitosis Cells Cultured biology Cell growth Chemistry Molecular biology Extracellular Matrix medicine.anatomical_structure Proteoglycan Biochemistry Protein Biosynthesis biology.protein Cattle Fibroblast Growth Factor 2 Proteoglycans Collagen Electron microscope Gels Cell Division |
| Zdroj: | Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 20(5) |
| ISSN: | 0736-0266 |
| Popis: | Low cell density cell numbers and dedifferentiation are two major problems of human chondrocyte culture associated with articular cartilage repair. Bovine chondrocytes seeded at low density (3.5 x 10(4) cells/ml of gels) in three-dimensional collagen type I gels do proliferate and maintain their phenotype as shown by cell counts, morphology and matrix synthesis. The combination of three growth factors (3GFs; 10 ng/ml TGF-beta1 + 100 ng/ml IGF-I + 10 ng/ml b-FGF) added to serum-free culture medium in this culture system enhances the mitotic activity of bovine chondrocytes similar to 20% foetal calf serum (FCS). At day 21, cells proliferated by 41 fold in gels-FCS and 37 fold in gels-3GFs. Protein synthesis by gels-3GFs cultures was similar to 20% FCS when cultured for 3 weeks but much less proteoglycan was synthesized. The matrix deposition as observed by light and electron microscopy was quite different. More small diameter branching collagen fibrils and a denser matrix were presented in gels-FCS culture whilst loosely arranged larger diameter collagen fibrils were observed in gels-3GFs. |
| Databáze: | OpenAIRE |
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