Electrophoretic analysis of near-isogenic avirulent and virulent lineages of the parthenogenetic root-knot nematode Meloidogyne incognita

Autor: A. Dalmasso, M.-N. Rosso, Michel Bongiovanni, Philippe Castagnone-Sereno
Přispěvatelé: Laboratoire de biologie des invertébrés, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration
Rok vydání: 1995
Předmět:
Zdroj: Physiological and Molecular Plant Pathology
Physiological and Molecular Plant Pathology, Elsevier, 1995, pp.293-302
ISSN: 0885-5765
1096-1178
DOI: 10.1006/pmpp.1995.1059
Popis: Soluble proteins from second-stage juveniles and females of two independently selected pairs of avirulent and virulent near-isogenic lines of the parthenogenetic root-knot nematode Meloidogyne incognita , both originating from a single individual, were compared by means of one- and two-dimensional polyacrylamide gel electrophoresis, and using a sensitive silver stain. Large proteic differences appeared to be correlated with the nematode development stage, mobile (i.e. juveniles) v. sedentary (i.e. females) stages. Electrophoretic analysis of avirulent and virulent individuals, independently from their developmental stage, showed a very high homology between their soluble protein contents, which strongly supports their near-isogenicity at the biochemical level. Two consistent differences were nevertheless detected between the two-dimensional protein patterns from females of the two genotypes: one protein, named pda , was present only in avirulent nematodes; a second one, named pdv + , was reproducibly found to be more abundant in virulent nematodes. The involvement of these two proteins as putative M. incognita (a) virulence factors is discussed.
Databáze: OpenAIRE
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