Aromatase activity in human adipose tissue stromal cells; the effect of fetal bovine serum fractions on dexamethasone-stimulated aromatization
| Autor: | C. Longcope, P. Lueprasitsakul |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Stromal cell Endocrinology Diabetes and Metabolism Clinical Biochemistry Ultrafiltration Adipose tissue Stimulation Biochemistry Dexamethasone Aromatase Endocrinology Internal medicine medicine Animals Humans Bovine serum albumin Molecular Biology Cells Cultured biology Chemistry fungi Androstenedione Cell Biology respiratory system equipment and supplies Blood Adipose Tissue Sephadex Chromatography Gel biology.protein Molecular Medicine Cattle sense organs Fetal bovine serum medicine.drug |
| Zdroj: | The Journal of Steroid Biochemistry and Molecular Biology. 39:353-357 |
| ISSN: | 0960-0760 |
| DOI: | 10.1016/0960-0760(91)90046-8 |
| Popis: | Aromatization in human adipose stromal cells is stimulated by dexamethasone, but only in the presence of fetal bovine serum (FBS). To determine whether there was a specific fraction of FBS responsible for this stimulation, FBS was fractionated either by a pressure-driven ultrafiltration membrane or by Sephadex gel filtration techniques. The stimulating factor(s) appeared to be in the FBS fraction of 150,000-300,000 Mw by Sephadex filtration. Conversely, FBS fractions with less than 30,000 Mw as separated by the former method inhibited the dexamethasone-stimulated aromatization of cultured adipose stromal cells. Bovine serum albumin, which constituted the major portion of FBS, had no discernible effect on the dexamethasone action on the aromatization of these cells. |
| Databáze: | OpenAIRE |
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