Dual-functional protein for one-step production of a soluble and targeted fluorescent dye
| Autor: | Yunjie Xiao, Yanyan Wang, Qian Zhang, Bin Wang, Shuxian Meng, Zefang Wang, Fengnan Sun, Yaqing Feng, Ziyu Han, Haitao Yang |
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| Rok vydání: | 2018 |
| Předmět: |
Infrared Rays
Hydrophobin Porphobilinogen Recombinant Fusion Proteins Sonication Mice Nude Medicine (miscellaneous) Antineoplastic Agents Peptide 02 engineering and technology 010402 general chemistry 01 natural sciences Mice chemistry.chemical_compound Nanocages Nanocapsules protein nanocage Cell Line Tumor Neoplasms Animals Pharmacology Toxicology and Pharmaceutics (miscellaneous) Boron Fluorescent Dyes chemistry.chemical_classification Microscopy Confocal NIR fluorescent probes Imidazoles self-assembly Neoplasms Experimental RGD peptide Flow Cytometry Integrin alphaVbeta3 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Solubility chemistry Biophysics Female Self-assembly BODIPY 0210 nano-technology Selectivity Oligopeptides Research Paper |
| Zdroj: | Theranostics |
| ISSN: | 1838-7640 |
| DOI: | 10.7150/thno.24613 |
| Popis: | Low water solubility and poor selectivity are two fundamental limitations that compromise applications of near-infrared (NIR) fluorescent probes. Methods: Here, a simple strategy that can resolve these problems simultaneously was developed by using a novel hybrid protein named RGD-HFBI that is produced by fusion of hydrophobin HFBI and arginine-glycine-aspartic acid (RGD) peptide. This unique hybrid protein inherits self-assembly and targeting functions from HFBI and RGD peptide respectively. Results: Boron-dipyrromethene (BODIPY) used as a model NIR dye can be efficiently dispersed in the RGD-HFBI solution by simple mixing and sonication for 30 min. The data shows that self-assembled RGD-HFBI forms a protein nanocage by using the BODIPY as the assembly template. Cell uptake assay proves that RGD-HFBI/BODIPY can efficiently stain αvβ3 integrin-positive cancer cells. Finally, in vivo affinity tests fully demonstrate that the soluble RGD-HFBI/BODIPY complex selectively targets and labels tumor sites of tumor-bearing mice due to the high selectivity of the RGD peptide. Conclusion: Our one-step strategy using dual-functional RGD-HFBI opens a novel route to generate soluble and targeted NIR fluorescent dyes in a very simple and efficient way and may be developed as a general strategy to broaden their applications. |
| Databáze: | OpenAIRE |
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