Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores
| Autor: | R. Bizzarri, Ilaria Elena Palamà, Giovanna Barbarella, Massimo Baroncini, Giuseppe Gigli, Andrea Barbieri, Alessandro Bongini, F. Di Maria |
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| Přispěvatelé: | Di Maria, F., Palama, I. E., Baroncini, M., Barbieri, A., Bongini, A., Bizzarri, R., Gigli, Giuseppe, Barbarella, G., DI MARIA, FRANCESCA GIULIA, Palamã , I. E., Baroncini, Massimo, Bongini, Alessandro, Gigli, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cytoplasm
CHEMICAL TAGS Globular protein Cell Survival media_common.quotation_subject education Cell Fluorescent Dye Thiophenes Biochemistry Fluorescence Mice Thiophene medicine Animals Viability assay Physical and Theoretical Chemistry LIVING CELLS Internalization NIH 3T3 Cell FLUORESCENT-PROBES media_common Fluorescent Dyes chemistry.chemical_classification Staining and Labeling Animal Protein Organic Chemistry ESTERS Proteins ORGANIC-SYNTHESIS Fibroblasts Embryonic stem cell Staining Cell biology Cytosol medicine.anatomical_structure chemistry QUANTUM-DOT NIH 3T3 Cells Fibroblast |
| Zdroj: | Organic & biomolecular chemistry 12 (2014): 1603–1610. doi:10.1039/c3ob41982g info:cnr-pdr/source/autori:Di Maria F.[ 1,2 ] ; Palama I.E.[ 3 ] ; Baroncini M.[ 4 ] ; Barbieri A.[ 2 ]; Bongini A.[ 4 ]; Bizzarri R.[ 5 ]; Gigli G.[ 4,6 ]; Barbarella G.[ 2,7 ]/titolo:Live cell cytoplasm staining and selective labeling of intracellular proteins by non-toxic cell-permeant thiophene fluorophores/doi:10.1039%2Fc3ob41982g/rivista:Organic & biomolecular chemistry/anno:2014/pagina_da:1603/pagina_a:1610/intervallo_pagine:1603–1610/volume:12 |
| DOI: | 10.1039/c3ob41982g |
| Popis: | A structurally correlated series of cell-permeant thiophene fluorophores, characterized by intense green or red fluorescence inside live mouse embryonic fibroblasts, was developed. The fluorophores displayed rapid internalization, excellent retention inside the cells, and high optical stability in the cytosolic environment and did not alter cell viability and reproducibility. Depending on the molecular structure, they experienced distinct fate inside the cells: from bright and lasting staining of the cytoplasm to selective tagging of a small set of globular proteins. This journal is © The Royal Society of Chemistry 2014. |
| Databáze: | OpenAIRE |
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