Preventive effect of agomelatine in lipopolysaccharide-induced pancreatic pathology
| Autor: | Senay Topsakal, Ozlem Ozmen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
pancreas disease
Lipopolysaccharides Pathology Lipopolysaccharide Health Toxicology and Mutagenesis lipopolysaccharide-induced sepsis pathologist Lipopolysaccharide (LPS) 010501 environmental sciences Wistar rat Toxicology 01 natural sciences sirtuin 1 chemistry.chemical_compound 0302 clinical medicine caspase 8 biochemical analysis pancreas islet cell Acetamides mesenchyme cell rat animal pancreas glucose amylase blood level lipopolysaccharide single drug dose neutrophil acetamide derivative General Medicine haptoglobin medicine.anatomical_structure female immunohistochemistry histopathology Immunohistochemistry blood sampling prophylaxis Pancreas agomelatine morphometry medicine.drug endocrine pancreas medicine.medical_specialty amylase animal experiment interleukin 4 Article animal tissue Sepsis 03 medical and health sciences autopsy medicine Agomelatine triacylglycerol lipase blood level Animals controlled study Rats Wistar neutrophil chemotaxis pancreas hemorrhage protein expression 0105 earth and related environmental sciences Pharmacology Chemical Health and Safety nonhuman business.industry pancreas tissue animal model Public Health Environmental and Occupational Health pancreas cell medicine.disease triacylglycerol lipase Rats glucose blood level chemistry exocrine pancreas cell vacuole pathology interleukin 10 hyperemia business epithelium cell edema 030217 neurology & neurosurgery interstitium |
| Popis: | The aim of this study was to examine pancreatic lesions and the possible prophylactic effects of agomelatine (AGO) in lipopolysaccharide (LPS)-induced sepsis in rats. Twenty-four female, 1-year-old Wistar albino rats were divided into three groups: group I (control), group II (study group; 5 mg/kg LPS i.p., single dose), and group III (treatment group; LPS + AGO, single dose p.o., 20 mg/kg AGO + 5 mg/kg LPS, 30 minutes after AGO treatment). The rats were sacrificed six hours after LPS administration. At the necropsy, blood and pancreatic tissue samples were collected for biochemical, pathological, and immunohistochemical analyses. The results showed that LPS caused an increase in serum amylase and lipase levels and a decrease in glucose levels. Histopathological analysis revealed infiltration of numerous neutrophils in pancreatic interstitial tissue and in vessels. In addition, slight vacuoles indicating degenerative changes were observed in endocrine and exocrine pancreatic tissues. Increased caspase-8, haptoglobin (Hp), IL-4, and IL-10 and decreased SIRT-1 expression was observed in both endocrine and exocrine parts of the pancreas in the LPS group. AGO ameliorated the biochemical, histopathological, and immunohistochemical findings. The present study results revealed that LPS-induced pancreatic damage to both endocrine and exocrine cells. In contrast, AGO had ameliorative effects on both biochemical and pathological findings in rats. © 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group. |
| Databáze: | OpenAIRE |
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