Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
| Autor: | Henrik Bringmann, Judith Besseling, Michal Turek |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Nervous system
General Immunology and Microbiology biology Nematode caenorhabditis elegans ved/biology General Chemical Engineering General Neuroscience ved/biology.organism_classification_rank.species Anatomy biology.organism_classification General Biochemistry Genetics and Molecular Biology Life stage Cell biology Neural activity chemistry.chemical_compound medicine.anatomical_structure Calcium imaging chemistry medicine Agarose Model organism Caenorhabditis elegans |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/52742 |
| Popis: | Behavior is controlled by the nervous system. Calcium imaging is a straightforward method in the transparent nematode Caenorhabditis elegans to measure the activity of neurons during various behaviors. To correlate neural activity with behavior, the animal should not be immobilized but should be able to move. Many behavioral changes occur during long time scales and require recording over many hours of behavior. This also makes it necessary to culture the worms in the presence of food. How can worms be cultured and their neural activity imaged over long time scales? Agarose Microchamber Imaging (AMI) was previously developed to culture and observe small larvae and has now been adapted to study all life stages from early L1 until the adult stage of C. elegans. AMI can be performed on various life stages of C. elegans. Long-term calcium imaging is achieved without immobilizing the animals by using short externally triggered exposures combined with an electron multiplying charge-coupled device (EMCCD) camera recording. Zooming out or scanning can scale up this method to image up to 40 worms in parallel. Thus, a method is described to image behavior and neural activity over long time scales in all life stages of C. elegans. |
| Databáze: | OpenAIRE |
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