The Effects of 2′,4′-Dihydroxy-6′-methoxy-3′,5′- dimethylchalcone from Cleistocalyx operculatus Buds on Human Pancreatic Cancer Cell Lines
| Autor: | Jeong Hyung Lee, Pham Thi Kim Lien, Bui Hoang Minh, Phuong Thao Tran, Yen Nhi Nguyen, Manh Hung Tran, Huynh Nhu Tuan, Ha Van Oanh, Quynh Mai Thi Ngo |
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| Rok vydání: | 2019 |
| Předmět: |
PANC-1
Chalcone Syzygium Pharmaceutical Science pPancreatic cancer Antineoplastic Agents Apoptosis Article Cleistocalyx operculatus Analytical Chemistry lcsh:QD241-441 2′ 4′-dihydroxy-6′-methoxy-3′ 5′-dimethylchalcone (DMC) 03 medical and health sciences chemistry.chemical_compound Chalcones 0302 clinical medicine lcsh:Organic chemistry Cell Line Tumor Pancreatic cancer Drug Discovery medicine Humans Propidium iodide Physical and Theoretical Chemistry Cytotoxicity Cell Proliferation 030304 developmental biology 0303 health sciences Molecular Structure biology Caspase 3 Plant Extracts Cell growth Organic Chemistry biology.organism_classification medicine.disease Molecular biology Pancreatic Neoplasms Gene Expression Regulation chemistry Chemistry (miscellaneous) 030220 oncology & carcinogenesis Cancer cell Molecular Medicine Biomarkers |
| Zdroj: | Molecules Volume 24 Issue 14 Molecules, Vol 24, Iss 14, p 2538 (2019) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24142538 |
| Popis: | 2′,4′-Dihydroxy-6’-methoxy-3′,5′-dimethylchalcone (DMC), a principal natural chalcone of Cleistocalyx operculatus buds, suppresses the growth of many types of cancer cells. However, the effects of this compound on pancreatic cancer cells have not been evaluated. In our experiments, we explored the effects of this chalcone on two human pancreatic cancer cell lines. A cell proliferation assay revealed that DMC exhibited concentration-dependent cytotoxicity against PANC-1 and MIA PACA2 cells, with IC50 values of 10.5 ± 0.8 and 12.2 ± 0.9 µM, respectively. Treatment of DMC led to the apoptosis of PANC-1 by caspase-3 activation as revealed by annexin-V/propidium iodide double-staining. Western blotting indicated that DMC induced proteolytic activation of caspase-3 and -9, degradation of caspase-3 substrate proteins (including poly[ADP-ribose] polymerase [PARP]), augmented bak protein level, while attenuating the expression of bcl-2 in PANC-1 cells. Taken together, our results provide experimental evidence to support that DMC may serve as a useful chemotherapeutic agent for control of human pancreatic cancer cells. |
| Databáze: | OpenAIRE |
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