Detection of Epstein-Barr virus and human T-cell lymphotropic virus type 1 in malignant nodal lymphoma, studied in Okinawa, a subtropical area in Japan
| Autor: | Tsuyoshi Miyakuni, Hiroshi Uezato, Jun-ichi Miyagi, K. Ohshima, Masato Masuda, Nobuyuki Takasu, Takayoshi Toda |
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| Rok vydání: | 2002 |
| Předmět: |
Pathology
medicine.medical_specialty Epstein-Barr Virus Infections Herpesvirus 4 Human Virus Integration Biology medicine.disease_cause Polymerase Chain Reaction Herpesviridae Virus Japan immune system diseases hemic and lymphatic diseases Internal medicine medicine Gammaherpesvirinae Humans Leukemia-Lymphoma Adult T-Cell Human T cell lymphotropic virus type 1 Lymph node In Situ Hybridization Human T-lymphotropic virus 1 Tropical Climate Hematology Lymphoma Non-Hodgkin DNA Neoplasm medicine.disease biology.organism_classification Epstein–Barr virus Virology HTLV-I Infections Lymphoma Clone Cells medicine.anatomical_structure DNA Viral RNA Viral Lymph Nodes |
| Zdroj: | International journal of hematology. 75(1) |
| ISSN: | 0925-5710 |
| Popis: | Formalin-fixed paraffin-embedded lymph node samples were collected from 100 cases of malignant nodal lymphoma documented in Okinawa in the period from 1973 through 1998. According to the new World Health Organization classification, 12 cases were Hodgkin’s lymphoma (HL). Eighty-eight cases of non-Hodgkin’s lymphoma (NHL) included 54 cases of T-cell type and 34 cases of B-cell type. Using polymerase chain reaction (PCR), Epstein-Barr virus (EBV) was detected in 11 cases (91.7%) of HL and in 57 cases (64.8%) of NHL, and human T-cell lymphotropic virus type 1 (HTLV-1) was detected in 23 cases (26.1%) of NHL. Clonal integration of HTLV-1 was detected in 10 (43.5%) of 23 HTLV-1 PCR-positive cases by the inverse PCR technique. The EBV-infected cells were detected by EBER-1 in situ hybridization in 11 (91.7%) of 12 HL cases and in 64 (72.7%) of 88 NHL cases. Irrespective of phenotype and tissue type of the malignant lymphoma, the rate of EBV-positive infection in Okinawa was higher than that in any other districts reported in Japan. This characteristic high rate of EBV-positive infection in Okinawa can be ascribed to various factors, such as racial and geographical differences. |
| Databáze: | OpenAIRE |
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