Role of Acinetobacter baylyi Crc in Catabolite Repression of Enzymes for Aromatic Compound Catabolism
| Autor: | Ulrike Gerischer, Tina Zimmermann, Tobias Sorg, Simone Y. Siehler |
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| Rok vydání: | 2009 |
| Předmět: |
Operon
RNA Stability Molecular Sequence Data Succinic Acid Catabolite repression Repressor Genetics and Molecular Biology Protocatechuate-3 4-Dioxygenase Biology Hydrocarbons Aromatic Models Biological Microbiology Enzyme Repression Bacterial Proteins Transcription (biology) Transcriptional regulation Amino Acid Sequence neoplasms Molecular Biology Derepression Acetic Acid Acinetobacter Gene Expression Profiling Wild type Gene Expression Regulation Bacterial 4-Hydroxybenzoate-3-Monooxygenase digestive system diseases Carbon Enzymes Repressor Proteins Biochemistry Sequence Alignment Gene Deletion |
| Zdroj: | Journal of Bacteriology. 191:2834-2842 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.00817-08 |
| Popis: | Here, we describe for the first time the Crc ( c atabolite r epression c ontrol) protein from the soil bacterium Acinetobacter baylyi . Expression of A. baylyi crc varied according to the growth conditions. A strain with a disrupted crc gene showed the same growth as the wild type on a number of carbon sources. Carbon catabolite repression by acetate and succinate of protocatechuate 3,4-dioxygenase, the key enzyme of protocatechuate breakdown, was strongly reduced in the crc strain, whereas in the wild-type strain it underwent strong catabolite repression. This strong effect was not based on transcriptional regulation because the transcription pattern of the pca-qui operon (encoding protocatechuate 3,4-dioxygenase) did not reflect the derepression in the absence of Crc. pca-qui transcript abundance was slightly increased in the crc strain. Lack of Crc dramatically increased the mRNA stability of the pca-qui transcript (up to 14-fold), whereas two other transcripts ( pobA and catA ) remained unaffected. p -Hydroxybenzoate hydroxylase activity, encoded by pobA , was not significantly different in the absence of Crc, as protocatechuate 3,4-dioxygenase was. It is proposed that A. baylyi Crc is involved in the determination of the transcript stability of the pca-qui operon and thereby effects catabolite repression. |
| Databáze: | OpenAIRE |
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