A Solid-Phase Protein Assay
| Autor: | Hendrikus Pas, Mw Sulter, Pieter Jan Coenraads, Gj Kloosterhuis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Cell Extracts
Keratinocytes Ovalbumin Biophysics Biotin Succinimides Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Biochemistry Chemistry Techniques Analytical chemistry.chemical_compound Microtiter plate Lowry protein assay Animals Humans Bicinchoninic acid assay Molecular Biology Bradford protein assay Horseradish Peroxidase Chromatography biology Microchemistry Fibrinogen Proteins Serum Albumin Bovine Cell Biology Avidin chemistry Biotinylation biology.protein Cattle Colorimetry Quantitative analysis (chemistry) |
| Zdroj: | Analytical Biochemistry, 211(2), 301-304. ACADEMIC PRESS INC ELSEVIER SCIENCE |
| ISSN: | 0003-2697 |
| Popis: | A solid-phase protein assay (SPA) for the determination of protein concentrations in the nanogram range is described. The technique is based on biotinylation of immobilized protein on the solid phase of a microtiter plate and quantitation of the protein-biotin complexes by peroxidase-coupled avidin. Compared to the routinely used Bradford and Lowry protein detection techniques, the described SPA assay is (depending upon the protein assayed) 1000 to 10,000 times more sensitive. Moreover, the SPA assay can be suitable for protein quantitation of samples containing agents which commonly interfere with the routinely used detection techniques. The SPA assay is a valuable addition to the Bradford and the Lowry techniques. Used in combination with an antigen-specific ELISA it gives a reliable ratio of specific versus total protein. |
| Databáze: | OpenAIRE |
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