Ultrasensitive Quantitation of Genomic Chimerism by Single-Molecule Molecular Inversion Probe Capture and High-Throughput Sequencing of Copy Number Deletion Polymorphisms
| Autor: | Adam Waalkes, Francesca Urselli, J. Lee Nelson, Stephen J. Salipante, David Wu, Kelsi Penewit, Sami B. Kanaan, Jerald P. Radich |
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| Rok vydání: | 2021 |
| Předmět: |
DNA Copy Number Variations
Genotype Chemistry Residual cancer Hematopoietic Stem Cell Transplantation High-Throughput Nucleotide Sequencing Regular Article Computational biology Genomics Molecular Inversion Probe Inversion (discrete mathematics) Chimerism DNA sequencing Pathology and Forensic Medicine Molecular Medicine Molecule Humans |
| Zdroj: | J Mol Diagn |
| ISSN: | 1943-7811 |
| Popis: | Genomic chimerism represents co-existing cells with different genotypes and has diagnostic significance in transplant engraftment monitoring, residual cancer detection, and other contexts. We previously described an approach to chimerism detection by interrogating variably present or absent genomic loci using single-molecule molecular inversion probes (smMIPs) and next-generation sequencing, which provided ultrasensitive limits of detection ( |
| Databáze: | OpenAIRE |
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