Activation of stress-activated protein kinase in osteoarthritic cartilage: evidence for nitric oxide dependence

Autor:	J. Rediske, Steven B. Abramson, Detcho A. Stoyanovsky, C. Koehne, Mukundan Attur, Robert R. Clancy, K. Iyama, Ashok R. Amin
Rok vydání:	2001
Předmět:	Cartilage Articular Male Programmed cell death MAP Kinase Kinase 4 Blotting Western Biomedical Engineering Nitric Oxide Synthase Type II Apoptosis Nitric Oxide Chondrocyte Nitric oxide chemistry.chemical_compound Chondrocytes Rheumatology medicine Animals Humans Orthopedics and Sports Medicine Protein kinase A Mitogen-Activated Protein Kinase Kinases biology Chemistry Superoxide Tumor Necrosis Factor-alpha Stress-activated protein kinase Nitric oxide Osteoarthritic cartilage JNK Mitogen-Activated Protein Kinases Osteoarthritis Knee Cell biology Nitric oxide synthase medicine.anatomical_structure Biochemistry biology.protein Cattle Female Signal transduction Nitric Oxide Synthase Cartilage Diseases Peroxynitrite Interleukin-1
Zdroj:	Osteoarthritis and Cartilage. 9(4):294-299
ISSN:	1063-4584
DOI:	10.1053/joca.2000.0388
Popis:	Objective We have demonstrated in bovine chondrocytes that nitric oxide (NO) mediates IL1 dependent apoptosis under conditions of oxidant stress. This process is accompanied by activation of c-Jun NH2-terminal kinase (JNK; also called stress-activated protein kinase). In these studies we examined activation of JNK in explant cultures of human osteoarthritic cartilage obtained at joint replacement surgery and we characterized the role of peroxynitrite to act as an upstream trigger.Design A novel technique to isolate chondrocyte proteins (
Databáze:	OpenAIRE
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