T cell response in acute motor axonal neuropathy
| Autor: | Luca Battistini, Francesca Notturno, Christina M. Caporale, B. Creati, Maria Teresa Cencioni, Vincenza Prencipe, Antonino Uncini |
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| Předmět: |
Adult
Cytotoxicity Immunologic Lipopolysaccharides Male Cellular immunity Immunology CD1 G(M1) Ganglioside CD8-Positive T-Lymphocytes Biology Guillain-Barre Syndrome Acute motor axonal neuropathy medicine.disease_cause Antibodies Immunophenotyping Antigens CD1 Campylobacter jejuni Interferon-gamma Young Adult Immune system Antigen Gangliosides Campylobacter Infections medicine Humans Immunology and Allergy Motor Neuron Disease Cells Cultured Aged Glycoproteins Motor Neurons Pharmacology Immunity Cellular Ganglioside Tumor Necrosis Factor-alpha Dendritic Cells Middle Aged medicine.disease Axons Coculture Techniques Tumor Necrosis Factor Receptor Superfamily Member 7 Molecular mimicry Case-Control Studies Acute Disease Female lipids (amino acids peptides and proteins) CD8 |
| Zdroj: | Scopus-Elsevier |
| Popis: | There is evidence that in the acute axonal motor neuropathy (AMAN) subtype of Guillain-Barré syndrome antibodies to gangliosides, produced through molecular mimicry by antecedent Campylobacter jejuni ( C. jejuni) infection, attack gangliosides expressed in human peripheral nerve axolemma, inducing a primary axonal damage. The aim of this study is to investigate whether the T cell response has a role in AMAN pathogenesis. We isolated monocytes from 4 healthy subjects and 5 AMAN patients with antecedent C. jejuni infection and antibodies to GM1 and/or GDla gangliosides. Immature dendritic cells expressing CD1 molecules cultured with autologous T cells were stimulated with 2 lipopolysaccharides (LPSs) extracted from C. jejuni strains containing GM1 and GDla-like structures and with GM1 and GDla. The T cell response to LPSs and to gangliosides was determined by measuring the release of IFN-γ and TNF-α. We observed a T cell response to both LPSs in controls and AMAN patients, whereas only AMAN patients showed T cell reactivity to gangliosides GM1 and GD1a with a tight correlation between T cell reactivity to the ganglioside and individual antibody responses to the same ganglioside. T cells responding to gangliosides were CD1c-restricted CD8 positive and CD27 negative. These findings indicate a contribution of cellular immunity in the pathogenesis of AMAN. A possible role for ganglioside-reactive T cells might be to facilitate the production of antibodies against gangliosides. |
| Databáze: | OpenAIRE |
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